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Review Article Volume 7 Issue 4
Reduction diameter of CaCO3 crystals by using poly acrylic acid might improve cellular uptake of encapsulated curcumin in breast cancer
Hanafy NAN, El-Kemary M, Leporatti S
Received: January 01, 1971 | Published: July 23, 2018
Citation: Hanafy NAN, El-Kemary M, Leporatti S. Reduction diameter of CaCO3 crystals by using poly acrylic acid might improve cellular uptake of encapsulated curcumin in breast cancer. J Nanomed Res. 2018;7(4):235-239. DOI: 10.15406/jnmr.2018.07.00193
Abstract
Although curcumin has many biological activities like antioxidant, anti-inflammatory, anti-tumorigenic anti-coagulant, anti-bacterial and anti-carcinogenic agents it is not widely used for cancer treatment because of its poor aqueous solubility, low adsorption, rapid metabolism and finally it is very sensitive to light. A strategy to encapsulate it in a nanosized delivery carrier is still needed. Since the rate of cellular uptake depends mainly on size and shape of nano-carrier. For this reason, our target is to produce polylectrolyte multilayer capsules depending on characteristic of template such as shape, size and charge. CaCO3 has been chosen as a template because there is no histological evidence for its toxicity after core removal with ethylene di-amine tetra-acetic acid (EDTA) and it is safer in handling than other templates. One important issue is how to get smaller CaCO3 template in range of nanosized scale bar while keeping spherical shape and non-toxic as main target during preparation. In this study, polyacrylic acid (PAA) as a biodegradable polymer was used for shape and size of CaCO3 control during fabrication. Zeta potential was used for identifying ionic properties of polymer adsorption after each coating layer. Curcumin was loaded into capsules after core removal and adsorption was measured by spectrophotometry. Cytotoxicity and cellular internalization were measured by using 3,-4,5 dimethyithiazol-2,5 diphenyl tetrazolium bromide (MTT) assays and transmitted light fluorescence microscopy in empty capsules and in curcumin-loaded capsules. Encapsulation of curcumin inside carrier doped with PAA has resolved two issues: PAA has improved the size and shape of carrier and the carrier could be used as container for curcumin encapsulation.
Keywords: curcumin, calcium carbonate, poly(acrylic)acid, cytotoxicity, nanosized delivery carrier, nanoparticles, chitosan, multilayer nanoparticles, poly (sodium 4-styrene-sulfonate), poly (allylamine hydrochloride), ethylene di-amine tetra-acetic acid, polyacrylic acid, dimethyithiazol-2,5 diphenyl tetrazolium bromide, Fetal Bovine Serum, Transmission Electron Microscopy, fluorescence isothiocyanate
©2018 Hanafy, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.