Mini Review Volume 1 Issue 3
1RMIT University, Australia
2CSIR Indian Institute of Chemical Technology, India
Correspondence: Sheshanath V Bhosale, School of Science, RMIT University, Australia
Received: July 19, 2017 | Published: September 13, 2017
Citation: Bhosale SV, Aljabri M, Hundal AA, et al. Development in the drug delivery tools that transport medically active biomolecules. Open Access J Sci. 2017;1(3):54-56. DOI: 10.15406/oajs.2017.01.00013
The drug-delivery tool that transport medically active molecules to diseased cells, in a precise manner, have grew much consideration in past decades. Supramolecular self-assembled systems plays an important role in nanotechnology, biotechnology, and regenerative medicine.1 Based on self-assembly approach various drug-delivery systems have been developed for example polymers,2 micelles,3 vesicles,4 nanoparticles5 and vesicular-supported particles.6 Among these developed systems, silica particles based delivery tool have become popular as biocompatible alternatives.7 Most sophisticated mesoporous silica nanoparticle (MSNs) have widely used8 due to their applicability to release drug molecule in controlled in particular cells using internal stimuli, such as pH9 and enzymes10 or external stimuli such as light,11 redox properties12 and temperature.13 The most discovered approaches to controlled drug-release were based on MSN carriers through surface functionalisation, with biomolecule responsive gates, pH- or photo-triggered release from hollow MSN.14 However, these methods are challenging, and suffer from limitations due to the low tissue-penetration-depth of light.15
Thus, there is a need to develop ideal transport system that should be designed to control the release of loaded drug to the target areas,16 thereby increasing its local concentration, bioavailability and prolonging its retention. To overcome these issues, recently, silica nanoparticles have generated a significant amount of interest because of their intrinsic properties as shown to be biocompatible alternatives.17 Based on their characteristic few sophisticated delivery systems have been developed and employed using mesoporous silica nanoparticles.18 However, these delivery systems have their own advantages, but they suffer from their own limitations, such as poor chemical or thermal elimination19 and also difficulties in controlling targeting and the well-organised release of the drug, as well as rapid elimination by the immune system.20
Keeping this in mind recently we prepared well-like cavity–so called ‘yoctowells (1yL=8nm3 that is, 10-24L),’ by two step self-assembly, firstly covalently bound porphyrin to the silica surface and bolaaphiphiles around the base porphyrins.21 For selection of porphyrin as a base component has two important advantageous, first is it can use as a handle while drug loading and drug release and second is penetration of particles in particular cells can be monitored easily with fluorescence of base porphyrin. Yoctowells are easy to prepare and can be tuned based on application one wishes.22‒27 We have used these yoctowells for various applications such as The most fascinating property of yoctowells is their ability to induce the formation of well-filling “nanocrystals” in dilute aqueous solutions, for example cyclic edge amphiphiles24 or neurotransmitters.25 Due to yoctowells unique rigid and hydrophobic nature have gifted outstanding abilities to selectively bind various guests entities.
Taking advantageous of yoctowell capability to encapsulate drug molecule, recently, we have demonstrate the use these hydrophobic wells as simple model systems for the encapsulation FDA approved anti-cancer Doxorubcin (DOX) biologically active molecules and their release was monitored by biological stimuli i.e. pH.27 Typically, two step-self assembled yoctowells were firstly functionalised with ammonium group produced positive rim at the top of the wells, upon addition of DOX 2 molecule, the positive rim used for capping of the yoctowells by addition of an anionic-porphyrin 3 by electrostatic interaction. After confirmation by UV-vis absorption and fluorescence spectroscopy, we then studied controlled release of the DOX and capping porphyrin 3 from the yoctowells by pH control. This system provide the first report of effectiveness of the sustain release of the DOX 2 molecule selectively from the yoctowells, offers prospective for development of a new generation of drug-delivery system for practical application. However, this developed yoctowell drug-delivery system relied on non-active silica nanoparticles, and has limitations for targeted drug delivery. To overcome this literature support our hypothesis to transport systems based on magnetic nanoparticles which can be ideal for the controlled release of loaded drug to target areas, thereby increasing its local concentration and bioavailability, and prolonging its retention. As human tissues are transparent to magnetic fields,28 thus use of magnetic field can be a good substitute for exact and targeted release of drugs.29
Knowing the capabilities of yoctowells, and the importance of targeted delivery of cancer drugs, herein, we transport system on magnetic silica nanoparticles and their application for encapsulation and pH-controlled release of drug molecules is verified (Figure 1).30‒35 To demonstrate the process of the responsive mechanised yoctowells, mitoxantrone (MTZ) was chosen for the release experiments. MTZ is from a member of the anthracycline antibiotic family, and is a powerful anticancer drug against different malignancies for example malignant tumours, several forms of leukemia, as well as ovarian and breast cancer.26 MTZ has major clinical value due to its apparent lower risk of cardiotoxic effects, as compared with DOX 2.36‒39
In summary, we demonstrate two novel approaches, first is preparation yoctowells on the magnetic silica nanoparticles for targeted delivery and reusable applications, and second is the encapsulation drug DOX or MTZ and their release by manipulating naturally occurring stimuli in vivo, that is, pH. Thus, we believe in future the usefulness of the sustained release of the DOX or MTZ molecule from yoctowells may provide potential tool for the development of a new generation of targeted drug-delivery systems. Thus, designer yoctowells, may act as tiny chemical reactors or alternatives in vivo drug-delivery systems by manipulating the interactions between drug molecules and the walls of yoctowell gaps and/or base porphyrin, and are thought to provide a useful supramolecular tool and could open new opportunities in the realm for targeted therapies.
S.V.B. (RMIT) acknowledges the Australian Research Council for funding through the Future Fellowship Scheme (FT110100152).
The author declares no conflict of interest.
©2017 Bhosale, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.