Primary squamous cell carcinoma of endometrium-a rare presentation

doi:10.15406/ogij.2018.09.00393

eISSN: 2377-4304

Obstetrics & Gynecology International Journal

Case Report Special Issue Reproductive System & Sexual Disorders

Primary squamous cell carcinoma of endometrium-a rare presentation

Kafil Akhtar,¹

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Binjul Juneja,¹ Ghazala Mehdi,² Seema Rashid³

¹Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, India
²Department of Pathology, College of Medicine for Girls, Dubai, UAE 3Obstetrics and Gynecology, Jawaharlal Nehru Medical College, India

Correspondence: Kafil Akhtar, Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh (U.P), India

Received: May 08, 2017 | Published: November 30, 2018

Citation: Akhtar K, Juneja B, Mehdi G, Rashid S. Primary squamous cell carcinoma of endometrium- a rare presentation. Obstet Gynecol Int J. 2018;9(6):492-494. DOI: 10.15406/ogij.2018.09.00393

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Abstract

Primary endometrial squamous cell carcinoma is an extremely rare tumor with unclear pathogenesis. We report a case of 55-year-old postmenopausal woman who presented with abdominal distention and blood-stained vaginal discharge for 9 months. Clinically, chronic pyometra was considered. Total abdominal hysterectomy with a bilateral salpingo-oophorectomy was performed and histopathological examination revealed a diagnosis of primary endometrial invasive squamous cell carcinoma with associated complete squamous cell metaplasia of glandular endometrium and coexisting multifocal squamous cell dysplasia, without involvement of the uterine cervix. The tumoral cells showed to be p16 negative and strongly p53 positive.

Keywords: endometrium, histopathology, immunohistochemistry, primary, squamous cell carcinoma

Introduction

Squamous cell carcinoma develops in the endometrium but is extremely rare. Only 64 cases of primary endometrial squamous cell carcinoma (PESCC) have been documented in the literature.^1–3 It is usually seen in postmenopausalwomen and is strongly associated with cervical stenosis, pyometra, chronic inflammation, nulliparity and icthyosis uteri. Fluhmannin 1928 devised a strictcriteria for the diagnosis of primary endometrial squamous cell carcinoma, which includes¹ no coexisting glandular carcinoma in the endometrium,² no connection between the tumor in the endometrium and the squamous epithelium of the cervix, and³ no primary squamous cell carcinoma of the cervix.⁴We report a case of primary endometrial squamous cell carcinoma in a 55-year-old postmenopausal woman, who presented with abdominal distention and blood-stained vaginal discharge.

Case summary

A 55-year-old multiparous, postmenopausal woman presented with the history of progressively increasing lower abdominal mass for last 9 months. The patient was diagnosed and treated for CIN³ 5 years back, but no foci of invasive carcinoma was identified in the cervix. On recent clinical examination, an abdominal mass of 28 weeks size was palpable in the hypogastric region. Per-speculum examination showed an atrophied cervix. On per-vaginal examination, a midline mass about 28 weeks size was felt. On ultrasonography, uterine cavity was enlarged and distended with fluid and exhibited atrophied cervix. Radiological impression was pyometra or haematometra. Preoperatively, other laboratory tests were within normal limits. Clinically, a diagnosis of pyometra was made.

A total abdominal hysterectomy with a bilateral salpingo-oophorectomy was performed. Grossly, the uterus was enlarged, 12×10×6 cm in size and the uterine cavity was markedly distended and filled with purulent material with atrophied cervix (Figure 1). Microscopic examination of multiple sections of the corpus uteri and cervix revealed total replacement of the entire glandular endometrial lining by squamous epithelium with associated dysplasia and invasive, focally keratinizing, squamous cells carcinoma, deeply invading myometrium (Figure2). Extensive sampling did not reveal normal glandular lining of the endometrial cavity. There was no cervical or serosal involvement by the lesion. Both fallopian tubes, ovaries, omentum and all sampled lymph nodes were negative for malignancy. The diagnosis of primary squamous cell carcinoma of endometrium was made. Immunohistochemistry of the tumoral cells showed p16 negativity (Figure 3) and strong nuclear p53 positivity (Figure 4). Adjuvant radiation therapy targeting the parametrial region, along with cisplatinchemosensitization was administered to the patient. She is doing well without any evidence of disease on 12 months of follow up period.

Figure 1 Grossly, the uterus was enlarged, 12×10×6 cm in size and the uterine cavity was markedly distended and filled with purulent material with atrophied cervix.

Figure 2 The solid tumor consists of nests of varying size with a squamous differentiation including distinct intercellular bridges and abortive keratinization. Haematoxylin and Eosin x 40X.

Figure 3 Clusters of squamoid cells with marked cytologicatypia. Haematoxylin and Eosin x 40X.

Figure 4 Immunohistochemistry shows strong p53 nuclear positivity of tumor cells. IHC p53 x 10X.

Discussion

Primary endometrial squamous cell carcinoma is extremely rare, with only 100 cases being reported in the literature, since the ﬁrst report in 1892 by Gebhard.^4,5Endometrial squamous cell carcinoma usually occurs in postmenopausal women, with mean age of presentation being 67 years.^3,4Given the rarity of PESCC, the etiology of the disease isunclear. Various authors have speculated that the etiologycould be related to differentiation of pluripotent endometrialprecursor cells, heterotopic cervical tissue, or even HPVinfection.^3–5There is a strong association with cervical stenosis, pyometra, chronic inflammation and nulliparity. It is believed that tumor may arise from ichthyosis uteri - a condition in which the endometrium is replaced by keratinized squamous epithelium.^5,6 The presence of squamous epithelium in the endometrium, variously termed ichthyosis uteri (a condition in which endometrium is replaced by keratinized squamous epithelium), leukoplakia epidermidization, psoriasis uteri, epidermoidheteroplasia, cholesteometra and indirect regenerative squamous metaplasia, has been described under a variety of conditions and is benign in the majority of cases,^7,8 Invasive squamous cell carcinoma of the endometrium is rare and is thought to arise by one of the two mechanisms: upward spread of a primary cervical lesion or transformation of reserve or stem cells positioned between the glandular basement membrane and the endometrial columnar epithelium. Invasive squamous cell carcinoma as a result of upward spread from the cervix is very unlikely, if the cervical lesion is intramucosal. In the present case, only CIN grade-III was identified in the cervix for which, the patient was already treated. Several studies have explored the immunophenotype and molecular alterations of PESCC. Immunohistochemical and molecular analysis have consistently demonstrated p14 (a growth suppressor gene) andandrogen receptor negativity.^9,10 Further, four out of ten diagnosed tumors were positive for p16, a tumor suppressor protein, that has been associated with malignancy-inducing human papillomaviruses in the female genital tract.^9,10 Our patient tested negative for high-risk HPV and p16 but positive for p53.

Given the rarity of this condition, there is no consensus for the best way to manage these patients with PESCC. A review of the literature yields sparse reporting of treatment data and associated outcome. No final treatment recommendation has been given so far; therapy usually consists of surgical hysterectomy with adnexectomy and radiotherapy in some cases.^11,12 In endometrial carcinoma, the presence of a malignant squamous cell component worsens the prognosis.¹²Although advanced stage disease usually confers a worse prognosis, Horn reports a case of Stage III PESCC with no evidence of disease 15.2 years after diagnosis.^13,14The prognosis of squamous cell carcinoma is related to the stage at diagnosis. In a review of reported cases, 80% of stage I patients survived whereas survival for patients with stage III disease was only 20%.^15,16 Although rare, diagnosis of primary squamous cell carcinoma of endometrium should be considered in a postmenopausal elderly female presenting with pyometra and cervical stenosis.^9,17Given the limited numbers of patients, it is not possible to draw conclusions regarding the optimal management. Diagnosis of this rare entity is based on careful pathologic review of the hysterectomy specimen. The underlying etiology or inciting factors leading to this condition, and the optimal treatment thereof, have yet to be determined.