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Research Article Volume 9 Issue 1
Expression profile of cerebellar proteins in Balb/c mice maintained on a high-carbohydrate diet and treated with glibenclamide
Isadora de Lima e Silva, Alexandre Azenha Alves de Rezende, Stêfany Bruno de Assis Cau, Luciana Karen Calábria
Received: May 28, 2026 | Published: June 16, 2026
Citation: Silva IL, Rezende AAA, Cau SBA, et al. Expression profile of cerebellar proteins in Balb/c mice maintained on a high-carbohydrate diet and treated with glibenclamide. Open Access J Sci. 2026;9(1):319-325. DOI: 10.15406/oajs.2026.09.00315
Abstract
The consumption of diets rich in refined carbohydrates is closely associated with rising obesity rates and metabolic disruptions, such as type 2 diabetes mellitus , which is commonly managed with glibenclamide. Emerging evidence suggests that the cerebellum may participate in metabolic regulation and respond to both dietary and pharmacological interventions. This study evaluated protein expression profiles in the cerebellum of 16 male Balb/c mice (n = 4 per group) subjected to a standard diet, refined carbohydrate-rich diet, glibenclamide treatment (20 mg/kg), or in combination, and the respective controls for eight weeks. Cerebellar supernatant samples were analyzed using biochemical techniques (SDS-PAGE) and in silico predictive assays. Eight distinct protein bands were identified (P1–P8). Glibenclamide administration down-regulated the expression of bands P1 and P3, whereas the high-carbohydrate diet up-regulated them (p < 0.05). Band P6 expression decreased significantly under both individual treatments (p < 0.05). Conversely, P7 expression decreased exclusively following glibenclamide treatment, while P8 expression was enhanced solely by the dietary intervention (p < 0.05). Functional annotation indicated that 28.2% of these predicted proteins are involved in DNA and RNA processing events. In conclusion, a high-carbohydrate diet and glibenclamide independently modulate the cerebellar proteome without exhibiting a significant associated or synergistic interaction.
Keywords: isocaloric diet, electrophoretic profile, Nervous system
©2026 Silva, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.