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Research Article Volume 9 Issue 2
Urine proteome changes in an α-synuclein transgenic mouse model of Parkinson’s disease
Lujun Li, Xuanzhen Pan, Ting Wang, Yuanrui Hua, Youhe Gao
Received: May 16, 2020 | Published: May 26, 2020
Citation: Li L, Pan X, Wang T, et al. Urine proteome changes in an ?-synuclein transgenic mouse model of Parkinson’s disease. MOJ Proteomics Bioinform. 2020;9(2):42-51. DOI: 10.15406/mojpb.2020.09.00277
Abstract
Urine accommodates more changes than other fluids, and it is a good source in the search for early sensitive biomarkers. The present study collected urine samples from 2-, 4-, 6-, 8- and 10-month-old α-synuclein transgenic mice. Based on data-independent acquisition (DIA) technology, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for quantitative analysis. Seventeen human homologous differential proteins were screened and compared with those in the urine of 2-month-old mice, and 9 proteins were related to Parkinson’s disease (PD). Formin-2, Splicing factor 3A subunit 1, and Isopentenyldiphosphate Delta-isomerase 1 changed continuously in months 6, 8 and 10. These experiments and analyses demonstrated that the urine proteome reflected the development of α-synuclein transgenic mice and provided clues for the early clinical diagnosis of PD.
Keywords: urine, proteome, parkinson’s disease, biomarker, animal models
©2020 Li, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.