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Research Article Volume 8 Issue 1
Molecular analysis of proteins of BMP15 gene in ruminants and non-ruminants
DS Gwaza, BS Bibinu, NI Dim
Department of Animal Breeding and Physiology, University of Agriculture, Nigeria
Received: February 06, 2019 | Published: February 28, 2019
Citation: Bibinu BS, Gwaza DS, Dim NI. Molecular analysis of proteins of BMP15 gene in ruminants and non-ruminants. MOJ Proteomics Bioinform. 2019;8(1):22-25. DOI: 10.15406/mojpb.2019.08.00262
Abstract
Bone morphogenetic protein 15 (BMP15) is a member of the transforming growth factorß (TGFß) superfamily that is expressed by oocytes and play key roles in granulose cell development and fertility in animal. It is a gene that has major effect on ovulation rate and fecundity leading to its classification as a fecundity gene with the name FecX. This study investigated the molecular genetic variation of proteins of BMP15 gene of cattle, sheep, goats, swine, and chicken with a view to providing relevant genetic information for breeding and selection programmes in the studied species using computational method. A total of thirty seven (37) BMP15 nucleotide sequences comprising goats (18), sheep (6), cattle (6), swine (4) and chicken (3) were retrieved from the GenBank. Sequence alignment, translation and comparison of the BMP15 gene of the various species were done with ClustalW using IUB substitution matrix, gap open penalty of 15 and gap extension penalty of 6.66.Theamino acid composition revealed the abundance of leucine amino acid in all the studied species, and the predicted 3D BMP15 protein structure aligned well with the template. The information emanating from this study may be important for chemist, drug developers, breeders and molecular biologists in the development of functional hypotheses about hypothetical proteins, improving phasing signals in crystallography, selecting sites for mutagenesis and thus help in marker-assisted selection (MAS) for high litter size productivity in various livestock species.
Keywords: BMP15, protein, variation, in-silico, livestock, hypotheses, genbank, amino acid, breeding, physico-chemical, leucine
©2019 Bibinu, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.