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Research Article Volume 8 Issue 1
Lack of association between the occurrence of ectopic pregnancy and variations in the TGFβ1 and E-Catherin genes
Nurida Aghayeva, Ali Benian, Ilhan Yaylim, Saime Turan, Akin Sevinc
Received: January 01, 1971 | Published: January 8, 2019
Citation: Aghayeva N, Benian A, Yayl?m ?, et al. Lack of association between the occurrence of ectopic pregnancy and variations in the TGF?1 and E-Catherin genes. MOJ Proteomics Bioinformatics. 2019;8(1):1-5 DOI: 10.15406/mojpb.2019.08.00258
Abstract
Purpose of the research: The aim of our study is to investigate the association between the impact of Transforming Growth Factor Beta-1 (TGFβ1) 29T/C and E-Cadherin160C/A polymorphisms and occurrence of ectopic pregnancy.
Materials and methods: Our study was formulated as a case–control study where the genotype status of 95 women with a history of ectopic pregnancy were compared with 97 post-menopausal controls with at least two pregnancies and no history of ectopic pregnancy, with regards to their TGFβ1 29 T/C and E- Cadherin -160C/A polymorphism status. All blood samples were collected by standard peripheral venous puncture in EDTA containing tubes. DNA was extracted from peripheral blood leukocytes using standard procedures. Genotype status of the samples in their TGFβ1 and E-Cadherin genes was determined by Polymerase Chain Reaction (PCR) using specific primers designed to specifically amplify wild- type sequence, and detected by endonuclease digestion coupled to electrophoretic determination of the sequences. The differences in genotype and allele frequencies between the cases and normal controls were compared using statistical analysis. The differences in TGFβ1 and E-Cadherin genotype and allele frequencies between the cases and normal controls were compared using the Chi-Square test. Statistical significance with a P value lower than 0.05 was considered significant. Statistical analysis was performed using the SPSS software.
Principal results: Our analysis excluded the association of TGFβ1 29T/C and E-Cadherin 160C/A polymorphism with the occurrence of ectopic pregnancy. In this study, we did not observe and significant difference between the cases and controls in relation to the polymorphism of the TGFβ1 gene 29 T ⁄C (P = 0.289 ) and E-Cadherin gene -160 C ⁄A (P = 0. 572). Similar conclusions were valid for the frequencies of other alleles as well.
Conclusion: Our study proved the lack of association between ectopic pregnancy and TGFβ1 29 T/C and E- Cadherin 160C/A polymorphisms. Based on our knowledge, this is the first report on the association of the TGFβ1 29 T/C and E-Cadherin 160C/A gene polymorphisms with ectopic pregnancy.
Keywords: ectopic pregnancy, gene polymorphisms, E-Cadherin, cytokines, blastocyst implantation, endometrium, proliferation, E-Cadherin 160 C/A gene, deoxyribonucleoside , Hardy–Weinberg equilibrium
©2019 Aghayeva, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.