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Review Article Volume 7 Issue 6
Determining the antibacterial effect of Leptadenia hastata extracts against Klebsielia pneumonia and Staphylococcus aureus
Benedict Samling
Received: January 01, 1971 | Published: December 18, 2018
Citation: Umaru IJ, Samling B. Determining the antibacterial effect of Leptadenia hastata extracts against Klebsielia Pneumonia and staphylococcus aureus. MOJ Proteomics Bioinformatics. 2018;7(6):176-178 DOI: 10.15406/mojpb.2018.07.00254
Abstract
Introduction: As the worldwide mortality rate is high due to the pathogens and especially because of the bacteria associated dysenteriae, antibacterial effect is well known from centuries these plant Leptadenia hastata can be used to annihilate klebsielia pneumonia and staphylococcus aureus.
Objective: The study aims to determining the antibacterial effect of Leptadenia hastata Stem-bark solvent extracts of hexane, dichloromethane, ethyl acetate, chloroform, and methanol against Klebsielia Pneumonia and Staphylococcus aureus.
Methods: The crude extracts were tested by disc method on nutrient agar medium; the bacterial was sub-cultured in a 10mL of broth, each in universal glass bottle for 16 hours inside an incubator equipped with shaker at 37°C. After 16 hours’ incubation, turbidity (optical density/OD) of the bacterial broth was measured by using UV mini spectrophotometer (model 1240 of Shimadzu brand), comparable to that of nutrient broth standard tube for further use. Measurement was performed at wavelength 575nm and the bacterial broth was ready to be used when its turbidity was between OD 0.6 to 0.9. Nutrient broth was used to adjust the turbidity until the desired value was obtained.
Result: The results of antibacterial sensitivity assay of the six concentration of the different solvent extract of Leptadenia hastata are presented in Table 2 and Table 3. The antibacterial activities were observed in various ways with zone of inhibition diameters ranging from 0.70±0.00mm to1.27±0.06mm for staphylococcus aureus and klebsielia pneumonia respectively among the six-concentration selected (25, 50, 100, 250, 500, and 1000 ppm).
Conclusion: It is investigated in present studies that Leptadenia hastata parts extracts can be utilized against the management of antibacterial diseases particularly Klebsielia Pneumonia and Staphylococcus aureus.
Keywords: klebsielia pneumonia, staphylococcus aureus, leptadenia hastata, metabolites, asclepediacea, hypertension, phytochemicals, pathogen, centrifug, dichloromethane, antibacterial growth
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