MOJ
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Research Article Volume 10 Issue 1
CRISPR construct covering homo sapiens APOL (1-6) genes
Akinseye Olanrewaju Roland, Ojomo Joan L, Ebenezer Morayo Ale, Gbadamosi Folawiyo I
Received: April 18, 2021 | Published: June 11, 2021
Abstract
Among the several design tools available for CRISPR/Cas9genome editing, E-CRISP web application was chosen to design single guide RNA (sgRNA) because it provides flexible output, enabling design of multiple libraries. Recently, two coding sequence variants in APOL1, which encodes a trypanolytic factor in humans and gorillas, have been implicated to associate with kidney disease which could result from inaccurate genome editing. There is need to design accurate library of guided RNA for not only APOL1 but also APOL 2-6 for CRISPR/Cas9 genome editing experiment through which one can apply their products in real life experiment.
Keywords: CRISPR/Cas9, sgRNA, APOL (1-6), library, gene editing
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