Journal of
eISSN: 2378-3184
Submit manuscript...
Research Article Volume 15 Issue 2
Properties of cellulase in the stomach of Tilapia Oreochromis niloticus
Akiko Yamada Taniguchi, Katsumi Takano
Received: May 08, 2026 | Published: May 14, 2026
Citation: Taniguchi AY, Takano K. Properties of cellulase in the stomach of Tilapia Oreochromis niloticus. J Aquac Mar Biol. 2026;15(2):69-73. DOI: 10.15406/jamb.2026.15.00443
Abstract
Cellulase isolated from the stomach of Tilapia Oreochromis niloticus was purified by ammonium sulfate precipitation, followed by α-cyclodextrin Sepharose 6B affinty chromatography, CM ion-exchange chromatography, polyex changer PBE94 chromato focusing and gel filtration. Cellulase was found to be a single band when examined by SDS- polyacrylamide gel electrophoresis. The purifications of cellulase was 55-fold from the crude extract. The cellulase showed the optimum activity at pH 3.0 and 45°C, and the stability at pH 3.0-5.0 and below 50°C. The molecular weight of the enzyme was 56,000 and its pI was 7.8. This enzyme was specifically found to be able to hydrolyze cellulose and agarose. The cellulase was strongly inhibited by Hg2+ and PCMB.
Keywords: cellulase, Tilapia Oreochromis niloticus, Purification, enzymatic properties, stomach
©2026 Taniguchi, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.