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Journal of
eISSN: 2572-8466

Applied Biotechnology & Bioengineering

Image Article Volume 5 Issue 3

Atomic force microscopy based infrared (AFM–IR) spectroscopy and nuclear resonance vibrational spectroscopy comparative study on malignant and benign human cancer cells and tissues under synchrotron radiation with the passage of time

Alireza Heidari

Faculty of Chemistry, California South University, USA

Correspondence: Alireza Heidari, Faculty of Chemistry, California South University, 14731 Comet St. Irvine, CA 92604, USA

Received: February 21, 2018 | Published: May 14, 2018

Citation: Heidari A. Atomic force microscopy based infrared (AFM–IR) spectroscopy and nuclear resonance vibrational spectroscopy comparative study on malignant and benign human cancer cells and tissues under synchrotron radiation with the passage of time. J Appl Biotechnol Bioeng. 2018;5(3):142-148. DOI: 10.15406/jabb.2018.05.00129

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In the current study, we have experimentally and comparatively investigated and compared malignant human cancer cells and tissues before and after irradiating of synchrotron radiation using Atomic Force Microscopy Based Infrared (AFM–IR) Spectroscopy and Nuclear Resonance Vibrational Spectroscopy. It is clear that malignant human cancer cells and tissues have gradually transformed to benign human cancer cells and tissues under synchrotron radiation with the passage of time (Figures 1 & 2).1–145 It should be noted that malignant human cancer cells and tissues were exposed under white synchrotron radiation for 30 days. Furthermore, there is a shift of the spectrum in all of spectra after irradiating of synchrotron radiation that it is because of the malignant human cancer cells and tissues shrink post white synchrotron irradiation with the passage of time. In addition, all of the figures are related to the same human cancer cells and tissues. Moreover, in all of the figures y–axis shows intensity and also x–axis shows energy (keV) (Figures 1 & 2).101–145

  • A

  • B

    Figure 1 Atomic force microscopy based infrared (AFM–IR) Spectroscopy analysis of malignant human cancer cells and tissues (A) before and (B) after irradiating of synchrotron radiation in transformation process to benign human cancer cells and tissues with the passage of time.1–145

  • A

  • B

    Figure 2 Nuclear resonance vibrational spectroscopy analysis of malignant human cancer cells and tissues (A) before and (B) after irradiating of synchrotron radiation in transformation process to benign human cancer cells and tissues with the passage of time.1–145

It can be concluded that malignant human cancer cells and tissues have gradually transformed to benign human cancer cells and tissues under synchrotron radiation with the passage of time (Figures 1 & 2).1–145 It should be noted that malignant human cancer cells and tissues were exposed under white synchrotron radiation for 30 days. Furthermore, there is a shift of the spectrum in all of spectra after irradiating of synchrotron radiation that it is because of the malignant human cancer cells and tissues shrink post white synchrotron irradiation with the passage of time. In addition, all of the figures are related to the same human cancer cells and tissues. Moreover, in all of the figures y–axis shows intensity and also x–axis shows energy (keV) (Figures 1 & 2).101–145

Acknowledgements

None.

Conflict of interest

The authors declare that there is no conflict of interest.

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  102. Alireza Heidari. Pros and Cons Controversy on Molecular Imaging and Dynamics of Double–Standard DNA/RNA of Human Preserving Stem Cells–Binding Nano Molecules with Androgens/Anabolic Steroids (AAS) or Testosterone Derivatives through Tracking of Helium–4 Nucleus (Alpha Particle) Using Synchrotron Radiation. Arch Biotechnol Biomed. 2017;1(1):67–100.
  103. Alireza Heidari. Visualizing Metabolic Changes in Probing Human Cancer Cells and Tissues Metabolism Using Vivo 1H or Proton NMR, 13C NMR, 15N NMR and 31P NMR Spectroscopy and Self–Organizing Maps under Synchrotron Radiation. SOJ Mater Sci Eng. 2017;5(2):1–6.
  104. Alireza Heidari. Cavity Ring–Down Spectroscopy (CRDS), Circular Dichroism Spectroscopy, Cold Vapour Atomic Fluorescence Spectroscopy and Correlation Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues with the Passage of Time under Synchrotron Radiation. Challenges Cancer Detect Ther. 2017;4(2):e001.
  105. Alireza Heidari. Laser Spectroscopy, Laser–Induced Breakdown Spectroscopy and Laser–Induced Plasma Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues with the Passage of Time under Synchrotron Radiation. Int J Hepatol Gastroenterol. 2017;3(4):79–84.
  106. Alireza Heidari. Time–Resolved Spectroscopy and Time–Stretch Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues with the Passage of Time under Synchrotron Radiation. Pharmacovigilance and Drug Safety. 2017;4(2):e001.
  107. Alireza Heidari. Overview of the Role of Vitamins in Reducing Negative Effect of Decapeptyl (Triptorelin Acetate or Pamoate Salts) on Prostate Cancer Cells and Tissues in Prostate Cancer Treatment Process through transformation of malignant prostate tumors into benign prostate tumors under synchrotron radiation. Open J Anal Bioanal Chem. 2017;1(1):21–26.
  108. Alireza Heidari. Electron Phenomenological Spectroscopy, Electron Paramagnetic Resonance (EPR) Spectroscopy and Electron Spin Resonance (ESR) Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues with the Passage of Time under Synchrotron Radiation. Austin J Anal Pharm Chem. 2017;4(3):1091.
  109. Alireza Heidari. Therapeutic Nanomedicine Different High–Resolution Experimental Images and Computational Simulations for Human Brain Cancer Cells and Tissues Using Nanocarriers Deliver DNA/RNA to Brain Tumors under Synchrotron Radiation with the Passage of Time Using Mathematica and MATLAB. Madridge J Nano Tech Sci. 2017;2(2):77–83.
  110. Alireza Heidari. A Consensus and Prospective Study on Restoring Cadmium Oxide (CdO) Nanoparticles Sensitivity in Recurrent Ovarian Cancer by Extending the Cadmium Oxide (CdO) Nanoparticles–Free Interval Using Synchrotron Radiation Therapy as Antibody–Drug Conjugate for the Treatment of Limited–Stage Small Cell Diverse Epithelial Cancers. Cancer Clin Res Rep. 2017;1(2):e001.
  111. Alireza Heidari. A Novel and Modern Experimental Imaging and Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues with the Passage of Time under White Synchrotron Radiation. Cancer Sci Res Open Access. 2017;4(2):1–8.
  112. Alireza Heidari. Different High–Resolution Simulations of Medical, Medicinal, Clinical, Pharmaceutical and Therapeutics Oncology of Human Breast Cancer Translational Nano Drugs Delivery Treatment Process under Synchrotron and X–Ray Radiations. J Oral Cancer Res. 2017;1(1):12–17.
  113. Alireza Heidari. Vibrational Decihertz (dHz), Centihertz (cHz), Millihertz (mHz), Microhertz (μHz), Nanohertz (nHz), Picohertz (pHz), Femtohertz (fHz), Attohertz (aHz), Zeptohertz (zHz) and Yoctohertz (yHz) Imaging and Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation. International Journal of Biomedicine. 2017;7(4):335–340.
  114. Alireza Heidari. Force Spectroscopy and Fluorescence Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues with the Passage of Time under Synchrotron Radiation. EC Cancer. 2017;2(5):239–246.
  115. Alireza Heidari. Photoacoustic Spectroscopy, Photoemission Spectroscopy and Photothermal Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues with the Passage of Time under Synchrotron Radiation. BAOJ Cancer Res Ther. 2017;3(3):45–52.
  116. Alireza Heidari. J–Spectroscopy, Exchange Spectroscopy (EXSY), Nuclear Overhauser Effect Spectroscopy (NOESY) and Total Correlation Spectroscopy (TOCSY) Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation. EMS Eng Sci J. 2017;1(2):6–13.
  117. Alireza Heidari. Neutron Spin Echo Spectroscopy and Spin Noise Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues with the Passage of Time under Synchrotron Radiation. Int J Biopharm Sci. 2017;(1):103–107.
  118. Alireza Heidari. Vibrational Decahertz (daHz), Hectohertz (hHz), Kilohertz (kHz), Megahertz (MHz), Gigahertz (GHz), Terahertz (THz), Petahertz (PHz), Exahertz (EHz), Zettahertz (ZHz) and Yottahertz (YHz) Imaging and Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation. Madridge J Anal Sci Instrum. 2017;2(1):41–46.
  119.  Alireza Heidari. Two–Dimensional Infrared Correlation Spectroscopy, Linear Two–Dimensional Infrared Spectroscopy and Non–Linear Two–Dimensional Infrared Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation with the Passage of Time. J Mater Sci Nanotechnol. 2018;6(1):101.
  120. Alireza Heidari. Fourier Transform Infrared (FTIR) Spectroscopy, Near–Infrared Spectroscopy (NIRS) and Mid–Infrared Spectroscopy (MIRS) Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation with the Passage of Time. Int J Nanotechnol Nanomed. 2018;3(1):1–6.
  121. Alireza Heidari. Infrared Photo Dissociation Spectroscopy and Infrared Correlation Table Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation with the Passage of Time. Austin Pharmacol Pharm. 2018;3(1):1011.
  122. Alireza Heidari. Novel and Transcendental Prevention, Diagnosis and Treatment Strategies for Investigation of Interaction among Human Blood Cancer Cells, Tissues, Tumors and Metastases with Synchrotron Radiation under Anti–Cancer Nano Drugs Delivery Efficacy Using MATLAB Modeling and Simulation. Madridge J Nov Drug Res. 2018;1(1):18–24.
  123. Alireza Heidari. Comparative Study on Malignant and Benign Human Cancer Cells and Tissues with the Passage of Time under Synchrotron Radiation. Open Access J Trans Med Res. 2018;2(1):26–32.
  124. Marcia R, Ricardo G, Alireza H. Planting of Jaboticaba Trees for Landscape Repair of Degraded Area. Landscape Architecture and Regional Planning. 2018:3(1):1–9.
  125. Alireza Heidari. Fluorescence Spectroscopy, Phosphorescence Spectroscopy and Luminescence Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation with the Passage of Time. SM J Clin Med Imaging. 2018;4(1):1018.
  126. Alireza Heidari. Nuclear Inelastic Scattering Spectroscopy (NISS) and Nuclear Inelastic Absorption Spectroscopy (NIAS) Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation. Int J Pharm Sci. 2018;2(1):1–14.
  127. Alireza Heidari. X–Ray Diffraction (XRD), Powder X–Ray Diffraction (PXRD) and Energy–Dispersive X–Ray Diffraction (EDXRD) Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation. J Oncol Res. 2018;2(1):1–14.
  128. Alireza Heidari. Correlation Two–Dimensional Nuclear Magnetic Reso¬nance (NMR) (2D–NMR) (COSY) Imaging and Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation. EMS Can Sci. 2018;1(1):1.
  129. Alireza Heidari. Thermal Spectroscopy, Photothermal Spectroscopy, Thermal Microspectroscopy, Photothermal Microspectroscopy, Thermal Macrospectroscopy and Photothermal Macrospectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues with the Passage of Time under Synchrotron Radiation. SMJ Biometrics Biostat. 2018;3(1):1024.
  130. Alireza Heidari. A Modern and Comprehensive Experimental Biospectroscopic Comparative Study on Human Common Cancers’ Cells, Tissues and Tumors before and after Synchrotron Radiation Therapy. Open Acc J Oncol Med. 2018;1(1).
  131. Alireza Heidari. Heteronuclear Correlation Experiments such as Heteronuclear Single–Quantum Correlation Spectroscopy (HSQC), Heteronuclear Multiple–Quantum Correlation Spectroscopy (HMQC) and and Heteronuclear Multiple–Bond Correlation Spectroscopy (HMBC) Comparative Study On Malignant and Benign Human Endocrinology and Thyroid Cancer Cells and Tissues Under Synchrotron Radiation. Journal of Endocrinology and Thyroid Researc. 2017;3(1):555603.
  132. Alireza Heidari. Nuclear Resonance Vibrational Spectroscopy (NRVS), Nuclear Inelastic Scattering Spectroscopy (NISS), Nuclear Inelastic Absorption Spectroscopy (NIAS) and Nuclear Resonant Inelastic X–Ray Scattering Spectroscopy (NRIXSS) Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation. Int J Bioorg Chem Mol Biol. 2018;6(1):1–5.
  133. Alireza Heidari. A Novel and Modern Experimental Approach to Vibrational Circular Dichroism Spectroscopy and Video Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues with the Passage of Time under White and Monochromatic Synchrotron Radiation. Glob J Endocrinol Metab. 2018;1(3):514–519.
  134. Alireza Heidari. Pros and Cons Controversy on Heteronuclear Correlation Experiments such as Heteronuclear Single–Quantum Correlation Spectroscopy (HSQC), Heteronuclear Multiple–Quantum Correlation Spectroscopy (HMQC) and Heteronuclear Multiple–Bond Correlation Spectroscopy (HMBC) Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation. EMS Pharma J. 2018;1(1):2–8.
  135. Alireza Heidari. A Modern Comparative and Comprehensive Experimental Biospectroscopic Study on Different Types of Infrared Spectroscopy of Malignant and Benign Human Cancer Cells and Tissues with the Passage of Time under Synchrotron Radiation. J Analyt Molecul Tech. 2018;3(1):1–8.
  136. Alireza Heidari. Investigation of Cancer Types Using Synchrotron Technology for Proton Beam Therapy: An Experimental Biospectroscopic Comparative Study. European Modern Studies Journal. 2018;2(1):13–29.
  137. Alireza Heidari. Saturated Spectroscopy and Unsaturated Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues with the Passage of Time under Synchrotron Radiation. Imaging J Clin Medical Sci. 2018;5(1):1–7.
  138. Alireza Heidari. Small–Angle Neutron Scattering (SANS) and Wide–Angle X–Ray Diffraction (WAXD) Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation. Int J Bioorg Chem Mol Biol. 2018;6(2):1–6.
  139. Alireza Heidari. Investigation of Bladder Cancer, Breast Cancer, Colorectal Cancer, Endometrial Cancer, Kidney Cancer, Leukemia, Liver, Lung Cancer, Melanoma, Non–Hodgkin Lymphoma, Pancreatic Cancer, Prostate Cancer, Thyroid Cancer and Non–Melanoma Skin Cancer Using Synchrotron Technology for Proton Beam Therapy: An Experimental Biospectroscopic Comparative Study. Ther Res Skin Dis. 2018;1(1).
  140. Alireza Heidari. Attenuated Total Reflectance Fourier Transform Infrared (ATR–FTIR) Spectroscopy, Micro–Attenuated Total Reflectance Fourier Transform Infrared (Micro–ATR–FTIR) Spectroscopy and Macro–Attenuated Total Reflectance Fourier Transform Infrared (Macro–ATR–FTIR) Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation with the Passage of Time. International Journal of Chemistry Papers. 2018;2(1):1–12.
  141. Alireza Heidari. Mössbauer Spectroscopy, Mössbauer Emission Spectroscopy and 57Fe Mössbauer Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation. Acta Scientific Cancer Biology. 2018;2(3):17–20.
  142. Alireza Heidari. Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation with the Passage of Time. Organic & Medicinal Chem IJ. 2018;6(1):555676.
  143. Alireza Heidari. Correlation Spectroscopy, Exclusive Correlation Spectroscopy and Total Correlation Spectroscopy Comparative Study on Malignant and Benign Human AIDS–Related Cancers Cells and Tissues with the Passage of Time under Synchrotron Radiation. Int J Bioanal Biomed. 2018;2(1):1–7.
  144. Alireza Heidari. Biomedical Instrumentation and Applications of Biospectroscopic Methods and Techniques in Malignant and Benign Human Cancer Cells and Tissues Studies under Synchrotron Radiation and Anti–Cancer Nano Drugs Delivery. Am J Nanotechnol Nanomed. 2018;1(1):1–9.
  145. Alireza Heidari. Vivo 1H or Proton NMR, 13C NMR, 15N NMR and 31P NMR Spectroscopy Comparative Study on Malignant and Benign Human Cancer Cells and Tissues under Synchrotron Radiation. Ann Biomet Biostat. 2018;1(1):1001.
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