Submit manuscript...
International Journal of
eISSN: 2381-1803

Complementary & Alternative Medicine

Opinion Volume 9 Issue 3

Replacing Chemical Nematicides Using Biological Tools

Ishwar Prakash Sharma

Patanjali Herbal Research Department, Patanjali Research Institute, India

Correspondence: Ishwar Prakash Sharma, Patanjali Herbal Research Department, Patanjali Research Institute, Haridwar-249 405, Uttarakhand, India, Tel +91 7579095587

Received: October 30, 2017 | Published: November 14, 2017

Citation: Sharma IP (2017) Replacing Chemical Nematicides Using Biological Tools. Int J Complement Alt Med 9(3): 00297. DOI: 10.15406/ijcam.2017.09.00297

Download PDF


A major global challenge in the coming years will be to ensure food security and to feed the increasing human population. Now, we need to increase the sustainability of agricultural productivity to meet the increasing demand for food. According to Indian Agriculture Report 2012-2013, India has about 2.4% and 4% of the world’s geographical area and water resources respectively, but has to support about 17% of the world’s human population and 15% of the livestock. Agriculture is an important sector of the Indian economy, accounting for 14% of the nation’s gross domestic product (GDP), about 11% of its exports. Root-knot disease caused by root-knot nematode is an important factor which declines our crop yields, particularly in tomato it reduces more than 27% yield annually.

Nematicides of chemical origin are widely used for the management of root-knot nematodes; large scale use of such chemical nematicides poses environmental hazards, besides being costly and uneconomical. Many bioinoculants are being used for controlling large numbers of soil borne diseases including the root-knot disease. Large numbers of microbes have been evaluated for controlling such diseases among these microbes Arbuscular mycorrhizal (AM) fungi and plant growth promoting rhizobacteria (PGPRs) have the greater potential for controlling it and hence they are one of the important biocontrol agents which could be used in the place of chemical nematides in agricultural fields. These organisms are economically and environmentally secure biocontrol tools against the soil borne pathogens. Generally all these organisms including root-knot nematodes live in the rhizosphere of the plants where they share the same niche. Root-knot nematodes affect all economically important characters of plants viz. yield, growth, hight, etc. This pathogen generally causes stress to the plants because of its damaging effect on root cells. AM and PGPRs affect the life cycle of nematodes because they compete with the nematode inside the root cells and provide some resistance to the plants.

To check the effect of both bioinoculants on the nematode parasitism, an experiment was conducted in which tomato plants selected as experimental plants [1]. In the beginning, tomato seeds were surface disinfected by immersion into 70% ethanol and 3% sodium hypochlorite followed by washing with sterile water. After sterilization, the seeds were sown in the autoclaved sand. For this two nurseries (one with mycorrhiza and other without mycorrhizal culture) were prepared. Mycorrhizal culture was inoculated during seeds sowing in nursery; a thin layer of mycorrhizal soil from maize rhizosphere was inoculated in the pure sterilized sand followed by sowing of sterilized tomato seeds in the inoculums. For non-mycorrhizal plants, the same nursery was prepared without mycorrhiza. Hoagland solution [2] was given weekly in both the nurseries. After four leaves stage the plants were transplanted into the 1:1 ratio sand and soil inoculated with the plant growth promoting rhizobacteria, fluorescent pseudomonad strains R62 and R81. Ten days after transplanting 500 freshly hatched nematode second stage juveniles (J2) were inoculated near the roots, the observations were taken after 30 days of the J2 inoculation.

Nematode induced parameters, numbers of root galls (49.92%, 33.67% and 29.82%), egg masses (51.72%, 27.01% and 16.09%), adult females (56.47%, 37.06% and 30%) and eggs per egg mass (64.13%, 33.89% and 25.53%) reduction were found in co-inoculation (AM and PGPR) followed by in AM and PGPR treated plants, respectively over nematode infected plants. All data have been represented in Figure 1. In nutshell, it may be concluded in this experiment that both of the biocontrol agents i.e. AM and PGPRs are useful for the management of the root-knot disease in the agricultural crops and also they act as an economically and environmentally secure biocontrol tool to replace the chemical nematicides. Hence, application of these biocontrol agents should be encouraged for better agricultural productivity and for sustaining a secured environment as well.

Figure 1: Effect of selected bioinoculents on nematode induced parameters.

C: Control; N: Nematodes; P: PGPRs; A: Arbuscular Mycorrhiza ‘AM’. Error bars represent standard error of three replicates. Different letters indicate a significant difference (P<0.05) of treatment.


  1. Sharma IP, Sharma AK (2017) Physiological and biochemical changes in tomato cultivar PT-3 with dual inoculation of mycorrhiza and PGPR. Symbiosis 71(3): 175-183.
  2. Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Calif Agr Expt Sta 347: 1-32.
Creative Commons Attribution License

©2017 Sharma. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.