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Research Article Volume 10 Issue 1
Antioxidant and Antiulcer Potential of Hydrolea Zeylanica (L.) Vahl against Gastric Ulcers in Rats
Atish Kumar Sahoo, Satish Kanhar
Phytotherapy Research Lab, Regional Plant Resource Centre, India
Received: October 28, 2017 | Published: December 14, 2017
Citation: Sahoo AK, Kanhar S (2017) Antioxidant and Antiulcer Potential of Hydrolea Zeylanica (L.) Vahl against Gastric Ulcers in Rats. Int J Complement Alt Med 10(1): 00324. DOI: 10.15406/ijcam.2017.10.00324
Abstract
Hydrolea zeylanica(L.) Vahl(Hydroleaceae) an aquatic, edible medicinal plant is useful in healing ulcer. Objective was to establish antioxidant principles in antiulcer activities of H. zeylanica in ulcer induced in rats. In order to explain the role of antioxidant principles in antiulcerogenic activities, in vitro 1, 1-diphenyl-2-picryl hydrazyl (DPPH), nitric oxide (NO), superoxide anion (SOD) and hydroxyl (OH) free radical scavenging activities of successive solvent extracted fractions of leaves were performed. Antiulcer activities of ethyl acetate fractions of H. zeylanica (HZEA, 300 and 400 mg/kg) were evaluated by performing diclofenac sodium and pylorus ligation induced ulcer in rats. Gastroprotective effects of HZEA were determined by assessing gastric volume, gastric-pH, free acidity, total acidity, ulcer index and % protection. Oxidative stress markers as MDA, GSH, CAT of gastric mucosa were evaluated. Histological studies were performed on stomach tissues. Antioxidant activities of HZEA were better than other fractions with IC50 of DPPH, SOD, NO and OH were 41.2±3.21, 59.71±1.45, 73.82±1.89, 48.91±0.89 µg/mL respectively. Total phenolic and flavonoid contents of HZEA were 286.33±9.16 mg GAeqv/g and 208.24±5.56mg Queqv/g. Gastric volume, free and total acidity, ulcerative index were significantly (p<0.01) reduced whereas gastric-pH and % protection were significant increased (p<0.01) in both models. There was a significant (p<0.01) reduction observed in MDA and increased in GSH, CAT levels in gastric tissues. Presence of phenols and flavonoids could be responsible for anti-ulcerative properties of HZEA.
Keywords: Hydrolea zeylanica, Antioxidant, Antiulcer, Diclofenac sodium, pyloric ligation, DPPH, Diphenyl-2-Picryl Hydrazyl, NO, Nitric Oxide, SOD, Superoxide Anion, OH, Hydroxyl, NSAIDs, Non-Steroidal Anti-Inflammatory Drugs, ROS, Reactive Oxygen Species, TBA, thiobarbituric acid, PMS, phenazine methosulphate, TPC, Total phenolic contents, GAE, gallic acid equivalents, TFC, Total flavonoid contents, SOD, Superoxide anion scavenger, NIN, National institute of nutrition
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