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eISSN: 2469-2778

Hematology & Transfusion International Journal

Review Article Volume 5 Issue 3

Pediatric acute lymphoblastic leukemia

Theodore Zwerdling

Department of Pediatrics, Wright Street Medical Center, USA

Correspondence: Theodore Zwerdling, Department of Pediatrics, Elica Health Centers, Wright Street Medical Center, 1750 Wright Street, Sacramento CA 95825, USA, Tel 855-354-2242, Fax 916-779-7561

Received: July 12, 2017 | Published: September 28, 2017

Citation: Zwerdling T. Pediatric acute lymphoblastic leukemia. Hematol Transfus Int J. 2017;5(3):232-238. DOI: 10.15406/htij.2017.05.00118

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Abstract

Progress in the understanding and treatment of pediatric acute lymphoblastic leukemia over the past 60 years has been remarkable and it is now expected that 80-85% of children diagnosed will be cured of this disease. This review article discusses risk factors, classification, presentation, risk groups, treatment, outcome, and long-term complications of therapy. Pediatric cancer studies serve as a model for national and international group cooperation to treat uncommon diseases.

Keywords: pediatric leukemia, review, key aspects, cooperative group participation

Abbreviations

BMF, berlin-muenster-frankfort; CNS, central nervous system; COG, children’s oncology group; CMV, cytomegalovirus; FAB, french-american-british; HIV, human immunodeficiency virus; MRD, minimal residual disease; pALL, pediatric acute lymphoblastic leukemia; SCT, stem cell transplantation; WBC, white blood cell; 6-MP, 6-mercaptopurine  

Introduction

This review will focus on pediatric acute lymphoblastic leukemia (pALL), highlighting risk factors, classification, presentation, risk group assignment and treatment stratification, treatment, outcome, and long-term consequences of therapy. Other forms of pediatric leukemia, such as acute and chronic myeloid leukemia are not reviewed. The reader is referred to several recent publications reviewing these topics.1–4 No discussion of pediatric leukemia would be complete without reference to the scientific principles and organizations which have taken this complex disease, largely fatal in the 1960’s, now mostly curable less than 60 years later.

Discussion

Background

pALL comprises a group of malignancies involving B- and T-lineage cells occurring from birth to age 21years (some protocols allow participation of patients up to age 30years). While the upper limit of age is somewhat arbitrary, biologic characteristics of these disorders change with increasing age. As will be discussed, age remains one initial variable defining risk stratification and prognosis.5 There are approximately 3,100 new cases of pALL in North America per year, a number which has been increasing over the past decades. pALL presents as a bimodal distribution from ~80 cases/million/year, ages 1-4years, to 24 cases/million/year, ages 10-14years. This disease effects caucasian children more commonly than other races.6,7

Early researchers realized that with small numbers of new patients and given the complexity of the disease process, little progress would be possible in terms of understanding and treatment by single institutional studies. Therefore, in the 1960’s, efforts were mounted to develop national co-operative groups charged with studying science, classification, treatment, and outcome of pALL. Currently the Children’s Oncology Group (COG) is responsible for providing state of the art protocols, data management and studying biology of pALL, now on an international scope.8 COG protocols, for all forms of childhood cancer, are developed by statisticians, nurses, physicians, researchers, psychologists, and many others who all play important roles in their formation and implementation. Other study groups perform similar functions in other parts of the world, such as the European Berlin-Muenster-Frankfort (BMF) group, and North America, for example, St. Jude Children’s Research Hospital.

Known risk factors involved in development of pALL

Risk factors for the vast majority of children and adolescents developing pALL remain mostly unknown at the present time. However, a few well characterized disorders can be associated with a higher risk of developing leukemia.9 For example, the most common, known genetic abnormality is Down syndrome or Trisomy 21. These children have a cumulative risk of approximately 2% by age 5years of developing leukemia.10,11 Several other genetic conditions which demonstrate a high incidence of pALL, have in common DNA abnormalities such as susceptibility to increased breakage, and abnormally functioning repair enzymes. Table 1 outlines some of these syndromes. Other risk factors include maternal and post-natal exposure to X-rays, and a history of chemotherapy.20–23

Disorder

Reference

Neurofibromatosis

12

Ataxic Telangiectasia

13

Li-Fraumeni Syndrome

9,14,15

Mismatch Repair Deficiency

16

Fanconi Anemia

17

Bloom Syndrome

18,19

Table 1 Genetic disorders associated with pALL

Classification

Early classification schema for pALL relied on criteria using the French-American-British (FAB) system, based on morphology and other patient characteristics. The FAB system is no longer used as better and more objective methodologies have become clinical standard for classification. These newer schema are based on immunologic analysis of leukemic cells using flow cytometry and molecularly based characteristics of cancer cells. The WHO recognizes the following distinct types of leukemia:24

B-lymphoblastic leukemia

  1. B-lymphoblastic leukemia with t(9;22)(q34.1;q11.2); BCR-ABL1.
  2. B-lymphoblastic leukemia with t(v;11q23.3); KMT2A rearranged.
  3. B-lymphoblastic leukemia with t(12;21)(p13.2;q22.1); ETV6-RUNX1.
  4. B-lymphoblastic leukemia: hyperdiploidy.
  5. B-lymphoblastic leukemia: hypodiploidy.
  6. B-lymphoblastic leukemia with t(5;14)(q31.1;q32.3); IL3-IGH.
  7. B-lymphoblastic leukemia with t(1;19)(q23;p13.3); TCF3-PBX1.
  8. Others

T-lymphoblastic leukemia

Others: A simplified classification separates these leukemias into precursor B-Cell and T-Cell leukemias. The majority of pALL is of B-lineage, which is the focus of this review. Immunophenotyping of leukemic blast cells delineates not only the cell lineage but also the degree of maturation. Precursor B-Cell blasts, which accounts for ~80% of pALL, are characterized by immuno-reactivity with monoclonal antibodies to CD19, HLA-DR surface receptors and the presence of cytoplasmic CD79a. CD10 is present on about 90% of precursor B-cell pALL and its absence is associated with a poor outcome.25 The presence of cytoplasmic immunoglobulin or heavy chain is termed pre-B-Cell pALL and finally, true B-cell pALL express surface immunoglobulin, also known as Burkitt leukemia, requiring different treatment.26,27 T-Cell pALL may be distinguished by cytoplasmic CD3 and CD7 and CD2. DNA-based testing is performed as routine part of patient care and is important for risk stratification. Ploidy has emerged as an important determinate of risk assessment and can be evaluated by measuring DNA content of cells (DNA index) or karyotyping. Hyperdiploidy (51 to 65 chromosomes/cell) is found in 25% of cases of precursor B-cell pALL and is associated with a favorable outcome of treatment.28,29 Other ploidy variations are known, including near-haploid (24 to 29 chromosomes/cell) and portents a poor outcome.30 Fluorescence in situ hybridization analysis can be of benefit for interphase or metaphase cell preparations. Using this technique, it has been found that trisomy 4 and 10 have a favorable outcome, for example.31

Table 2 illustrates some of the many translocations found in pALL and associated features. Other DNA analyses are important predictors of toxicity including polymorphisms of the gene responsible for metabolism of 6-mercaptopurine (6-MP), a commonly used treatment.36 Patients found to be homozygous for variant alleles of the TMTP gene tolerate much lower doses of 6-MP and must be dose reduced to prevent excessive toxicity. Other polymorphisms are known, but few have been tested sufficiently to direct therapy.

Translocation

Involved Genes

Prognostic
Significance

Notes/Reference

t(12;21(p13.2;q22.)

ETV6-RUNX1

Favorable

32,33

t(9;22)(q34.1;q11.2)

BCR-ABL1 (Ph+)

Favorable

Tyrosine kinase inhibitors;34

t(v;11q23.3)

MLL

Poor

Infants, high WBC 35

(1;19)(q23;p13.3)

TCF3-PBX1

Variable

27

t(8;14)(q24;32)

MYC-IgH

Good

26

Table 2 Common chromosomal translocations associated with pALL

Clinical presentation and evaluation

The clinical presentation of pALL relates to the signs and symptoms of bone marrow replacement by malignant blast cells and subsequent loss of normal cellular elements. These signs and symptoms typically include fatigue, appearing pale, bleeding (petechiae), adenopathy, hepatomegaly, splenomegaly and fevers. However, at other times patients present with fever, infections, complaints of bone pain, or inability to walk. Very young children or babies may present with irritability, poor appetite and weight loss. For children with central nervous system disease (CNS) cranial nerve abnormalities may be seen. T-Cell leukemia presents with difficulty breathing or wheezing, caused by an enlarged thymus gland and represents a pediatric emergency. Compression of the airway can result in life threatening respiratory failure and/or hypoxia. Primary care providers, as well as Emergency Department physicians, must recognize not all wheezing is due to asthma. Initiation of steroid therapy, a common practice for patients with asthma, can result in rapid tumor lysis, metabolic disturbances and death prior to start of therapy.

Some males will present with testicular masses, representing overt leukemic infiltration of the testes.37 A similar presentation in females with ovarian masses is much less common. With a clinical suspicion of leukemia, an extensive laboratory investigation is required prior to any therapy. Complete blood count with differential, metabolic panel, chest X-ray, urine analysis, and coagulation studies should all be performed. Additional studies include bone marrow aspiration and biopsy, and lumbar puncture to determine extent of CNS disease. Other studies may be necessary guided by individual patient requirements. Blood for infectious evaluation should be obtained and can include blood cultures and titers for viral infectious agents such as Cytomegalovirus (CMV), Human Immunodeficiency Virus (HIV) and others. If the patient presents with high-risk features, which will require future anthracycline therapy, consideration to performance of an echocardiogram should be given.

Blood analysis for immunophenotyping, chromosomal analysis, and molecular studies are now routine part of evaluating a child with leukemia. Some research protocols require additional blood, bone marrow and spinal fluid studies to be obtained prior to start of therapy. Research studies not needed for clinical care should be discussed with parents or guardians prior to their collection. Many patients are offered treatment according to research protocols, most of the time welcomed by parents/guardians, so it is important to talk with families, including the child, about this possibility to minimize delays in treatment or loss of research study participation which may be available.

Risk groups and treatment stratification

Risk group and treatment stratification involving children with pALL is an ongoing and evolving process. Early studies emphasized age, white blood cell (WBC) counts and involvement of the CNS. With wide spread availability of flow analysis and immunophenotyping, additional information yielded a better understanding of the disease and grouping of patients to receive more specific therapies. Chromosomal analysis and DNA studies further improved the classification of pALL. In the past decade, response to treatment has become a powerful indicator of the type of therapy an individual patient will derive the best risk to benefit ratio. Over the many research protocols correlating these variables and, linking uniform treatments to outcome, multiple risk strata are now defined. Patients may begin treatment in one risk group only to change to a different one as more information and their individual responses to treatment become known. This multi-tier, individualized approach allows directing higher risk patients to be treated more aggressively but sparing lower risk patients additional therapy which may not be needed. The following are some of the important indicators used to assign a patient to risk strata:

Initial risk classification

Age: this continues to be important for initial stratification with the most favorable group of children from 1 year to 10years. Very young (<1year) and teenage patients (>10years) do not have as favorable outcome.

WBC count: First WBC count, prior to any intervention is used for initial stratification with<50,000/cc favorable. Higher WBC count is associated with T-Cell leukemia and is a high risk feature.

Testicular involvement: Testicular involvement may present as a mass and it is controversial if this effects prognosis.37

CNS involvement: Greater than 5blast cells/cc or the presence of cranial nerve palsy/paralysis indicate overt disease. Formulas exist to correct for traumatic lumbar punctures.38,39

Light microscopy/immunophenotyping of blast cells: Morphology evaluation of leukemic cells confirms the diagnosis and usually rules out other forms of leukemia. Flow analysis, allows rapid immunophenotyping classification of the leukemia and is now possible with results available within 24hours. It is also a requirement for most research protocols.

Additional risk classification elements

DNA Studies: Ploidy, karyotype and DNA analysis for cryptic translocations results are also important to determine risk classification, results usually available during the first few weeks of treatment. These results may change risk category assignment. For example, the presence of a Ph+pALL is a high-risk factor and requires different therapies.

End of induction chemotherapy (4 weeks): With over 95% of children entering remission, remission status as an indicator of possible cure has been minimized (patients not entering remission at this time fare poorly). Additional response indicators, either more sensitive at end of induction or at earlier time points to identify risk categories were therefore needed.40,41

Minimal residual disease (MRD): Determinations of MRD, by flow cytometry analysis at several time points in therapy, are                now routine part of clinical practice and used to guide therapy. It is controversial at which time points and what space (peripheral blood or bone marrow) to best make use of MRD.41–49

Final risk group assignment is of importance as it allows rational assignment of patients to therapies designed to balance risks of therapy vs maximizing possibilities of cure.50 Chemotherapy remains highly toxic for these children not only while receiving treatment, but now equally important, for long-term effects. Based on this information patients are assigned to low, standard, high and very high risk categories. Different criteria are applied by the various co-operative groups to determine risk strata, and therefore, treatment. For example, the European Berlin-Muenster-Frankfort (BMF) group bases risk assignment on response to therapy by determining MRD at weeks 5 and 12.42,46 Only patients with Ph (+) and t(4;11) are considered high risk, irrespective of response. The COG combines other factors to define patient’s prognostic groups including age, WBC count, genetic abnormalities, MRD at various time points and absence of Down syndrome and pre-treatment steroids.41

Treatment principals

After completion of the diagnostic evaluation, and prior to initiation of chemotherapy, patients must be readied for treatment, which means several associated medical issues must be addressed. Many patients will have severe anemia and thrombocytopenia, problems treated with irradiated blood products. For patients presenting with fever, or other signs of infection, and low absolute neutrophil count, presumptive institution of broad spectrum antibiotics is warranted until culture results are known to be negative or infection treated. Placement of a central venous access devise is usually required to allow delivery of chemotherapy, procuring blood sampling, and to be used for instillation of anesthesia required for multiple lumbar punctures and bone marrow examinations. Patients should receive hydrating fluids and allopurinol to minimize metabolic complications associated with initiation of treatment and possible tumor lysis syndrome. Prophylaxis for Pneumocystis jirovecii is also begun prior to discharge. During the initial hospitalization significant resources are employed including nursing, social services, play therapists and others. These children are approached by a team to help educate and guide patients and parents/guardians through complex treatments and emotional concerns they may have.8 An example of treatment is shown in Table 3 for patients with standard risk pALL. Different treatments may be recommended based on the above defined risk strata.

Phase of Therapy

Duration

Treatments

Induction

4 Weeks

Vincristine, steroid, asparaginase,
intrathecal chemotherapy

Consolidation

4 Weeks

Vincristine, 6-mercaptopurine,
intrathecal chemotherapy

Interim Maintenance I

8 Weeks

Vincristine, methotrexate,
intrathecal chemotherapy

Delayed Intensification

8 Weeks

Vincristine, steroid, doxorubicin,
asparaginase, cyclophosphamide, 6-thioguanine,
cytarabine, intrathecal chemotherapy

Interim Maintenance II

8 Weeks

Vincristine, intrathecal chemotherapy

Maintenance

2 Years- Female,
3 Years- Male

Vincrisitne, steroid, 6-mercaptopurine,
intrathecal chemotherapy

Table 3 Example of standard risk treatment for pALL*

Treatment of relapsed pALL 

While most children and adolescents will be cured of disease undergoing current treatment plans, 5-20% are expected to relapse.50–53 Relapsed pALL is thus more common than many other cancers diagnosed in children and adolescents. The outcome for many of these children remains poor; however several characteristics have been identified, which in turn suggest therapeutic options. Site of relapse is important with patients having extra-medullary relapse fairing better than those with marrow involvement.54–57 Children relapsing early (<18 months from start of treatment) due particularly poorly while late relapses (>36months) have better outcomes. Patients with T-Cell disease also fair poorly.54 Using these prognostic factors, treatment consists of re-induction chemotherapy (4weeks) and determination of response. For patients with early relapse recommendations for Stem Cell Transplantation (SCT) using allogeneic or matched unrelated donors are considered.58–60 MRD should be minimal prior to SCT, even if it requires additional chemotherapy as higher residual disease portents SCT failure and recurrence of disease.61–63 For patients relapsing >18months into treatment, continuation of chemotherapy can be successful. Current cooperative group protocols are aimed at introducing novel drugs, such as Blinatumomab and Inotuzumab, in hopes of improving outcomes and also to evaluate their potential in upfront therapy.64,65

Most recently, the use of engineered T-Cells has entered clinical practice.66 In this study lentiviral transduced autologous T- cells were redirected to attack leukemic blasts which were CD19 positive, a common surface antigen on most pALL of B-lineage origin. Twenty-seven of 30 highly refractory patients went into remission, 67% lasting at least 6 months. Cytokine release syndrome occurred in 27% of patients and could be treated with an anti-IL-6 monoclonal antibody. Further trials of this new therapy will be needed to understand its place in the therapeutic armamentarium.

Long-term outcome for cured patients

With large numbers of children treated for and cured of pALL, further attention and emphasis has been placed on long-term consequences of treatment. For children 12years of age who are healthy and never had leukemia, life expectancy is about 88.4 years for males and 90.2years for females.67 Clearly, the long-term consequences are going to have a profound impact on the future of patients treated for leukemia. For example, the cumulative incidence of chronic medical problems, including death, for children treated for leukemia is approximately 20% by age 30 and over 50% at age 50, compared to non-treated siblings of 10% and 25%, respectively.68–70 Furthermore, these curves are not flat and the cumulative incidence continues to increase as these patients age.

Therefore long-term follow-up assumes an important part of medical care for these children, with problems reported for nearly every organ system. Table 4 highlights some of these ongoing health issues for this population. A comprehensive review of long-term health problems, and how to care for them, may be found at the COG web site and in several recent reviews.8,70–72 However important medical complications are, other social and societal factors arise as well. These patients may be denied insurance or required to pay higher premiums. There have been instances of discriminatory hiring practices making employment more difficult. Critical stages of normal childhood social development maybe interrupted and replaced with interactions typically occurring at older ages. Self esteem can be altered and effects on families, both social and economic, experienced. Finally, a growing problem for any child treated for cancer whether it is leukemia or other forms, relates to difficulties in finding health care providers after they transition to adult services. Specific programs to facilitate changing from pediatric to adult care models are now in place at larger institutions, but not available everywhere.73,74 It is known from other long-term follow-up studies that this population will have more medical needs as they age and require care provider’s who are able to recognize these and other yet unknown, complications of treatment.70

Organ System

Therapy Implied

Effect

Central Nervous
System

Vincristine

Neuropathy

Central Nervous
System

Methotrexate

Encephalopathy
Cognitive Dysfunction

Central Nervous
System

Radiation

Cognitive Dysfunction

Cardiovascular

Anthracycline/Radiation

Arrhythmia
Congestive Heart Failure

Pulmonary

Stem Cell Transplant

Bronchiolitis Obliterans

Gastrointestinal

Stem Cell Transplant
(Graft-vs-Host Disease)

Esophageal Strictures

Hepatic

Methotrexate

Hepatitis

Hepatic

6-mercaptopurine, 6-thioguanine

Hyperbilirubinemia
Fibrosis

Reproductive

Cyclophosphamide

Infertility

Renal

Cyclophosphamide

Renal Tubular Acidosis

Lympho-hematopoietic

Second malignancy

Table 4 Some long-term complications of treatment for pALL

Conclusion

The past 60years have seen remarkable improvements in understanding, treatment and, most importantly, survival for children and adolescents with pALL, yet much work remains.1,50 Curing these children and adolescents has come at a high price with respect to long-term sequela. Fully integrating these patients into society and allowing them to reach full potential seems to be a next goal for consideration. Additional inroads, working with our colleagues who care for adults, continue to be an important step and an ongoing challenge. Many individuals deserve recognition for their contributions to our current state of understanding and treatment, but none more so than the patients and their parents/guardians who have and continue participation in clinical trials. Well designed, prospective, randomized clinical trials remain the mainstay of progress and will likely be for the foreseeable future. Finally, can we speculate in the future better therapies will result in higher cure rates, with less toxicity and more tailored treatments? The answer is yes. And even more daring, can we see a time when we will be able to prevent this disease from ever happening?

Acknowledgements

I wish to acknowledge all the nurses, social workers, psychologists, play therapists, pharmacists, physicians, researchers, statisticians, and data managers all of whom contributed to this review. I am indebted to the patients, parents and/or guardians for their participation in research studies.

Conflict of interest

The author declares no conflict of interest.

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