eISSN: 2576-4462
Submit manuscript...
Research Article Volume 10 Issue 2
Herbaceous and woody cuttings of Cissus verticillata (princess vine)
Helena Flávia de Mello Pistune, Ana Maria Presaniuk Martins, Ricardo Antônio Ayub
Received: February 23, 2026 | Published: July 6, 2026
Citation: Pistune HFM, Martins AMP, Ayub RA, Midaoui ME, Maachi Y, et al. Herbaceous and woody cuttings of Cissus verticillata (princess vine). Horticult Int J. 2025;10(2):53-58. DOI: 10.15406/hij.2025.10.00358
Abstract
Cissus verticillata (L.) Nicolson & C.E. Jarvis, commonly known as princess vine, has been traditionally used to treat several diseases, particularly diabetes. This study evaluated different strategies for the ex-vitro vegetative propagation of C. verticillata. Stem cuttings approximately 5 cm long, each containing one axillary bud, were subjected to combinations of three factors: cutting type (herbaceous with one-half leaf or leafless woody), tube size (19 × 5.5 cm or 13 × 3.1 cm), and substrate (oxisol or organic substrate), totaling eight treatments. The cuttings were maintained in a greenhouse at 22 ± 2 °C and irrigated every two days. After 45 days, survival, rooting, shoot formation, callus formation, cutting establishment, root number, and root length were evaluated. The experiment followed a completely randomized 2 × 2 × 2 factorial design with ten replicates per treatment. Continuous variables were analyzed by analysis of variance or generalized linear models, whereas binary variables were analyzed using generalized linear models with a binomial distribution (p ≤ 0.05). Cissus verticillata exhibited high vegetative propagation capacity, with rooting rates ranging from 60 to 100%. Significant interactions between cutting type and tube size were observed for shoot formation and cutting establishment, indicating superior performance of herbaceous cuttings in larger containers and greater tolerance of woody cuttings to smaller ones. Callus formation was favored by the organic substrate, while the greatest root lengths were obtained in 19 × 5.5 cm tubes. The highest number of shoots was recorded in herbaceous cuttings grown in larger tubes filled with oxisol. These findings demonstrate the high potential of C. verticillata for ex vitro vegetative propagation and support the use of herbaceous cuttings in 19 × 5.5 cm tubes to maximize early seedling development and cutting establishment.
Keywords: medicinal plant, vegetative propagation, stem cuttings, substrate, nursery tube
©2026 Pistune, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.