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Biology and Medicine

Research Article Volume 1 Issue 4

Investigations of acute toxicity and neurotoxin effects of aqueous extracted pyrethroid (deltamethrin) from insecticide treated mosquito net on clarias gariepinus and heterobranchus bidorsalis

Lord Tertese Angahar

Department of Biological Sciences, Federal University of Agriculture, Nigeria

Correspondence: Lord Tertese Angahar, Department of Biological Sciences, Federal University of Agriculture P.M.B 2373 Makurdi, Nigeria

Received: June 21, 2017 | Published: July 10, 2017

Citation: Angahar LT. investigations of acute toxicity and neurotoxin effects of aqueous extracted pyrethroid (deltamethrin) from insecticide treated mosquito net on clarias gariepinus and heterobranchus bidorsalis. MOJ Biol Med. 2017;1(4):98–101. DOI: 10.15406/mojbm.2017.01.00020

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Abstract

There is dearth of literature on effects of aqueous extracted deltamethrin from Long-lasting Insecticide-treated Nets (LLINs) on fish. In malaria endemic regions LLINs are distributed free as governments’ efforts to control malaria. These mosquito nets are converted into fishing nets by fishermen. The aim of this study was to determine the effects of Aqueous Extracted Deltamethrin AED on Clarias gariepinusand Heterobranchus bidorsalis. Larva, fry and fingerlings stages of experimental fish species total 720 were exposed to 0, 50, 100, 150, 200, and 250ppt of AED for 24, 48, 72, and 96 hours. Guide for the care and use of experimental fish was followed. Data was analysed using ANOVA. 100% mortality was observed at 250ppt from both species at all stages in 48-96hours. Lowest lethal concentration LC was 50ppt for larva and fry stages of both species at 72 hours, and 100ppt at 96 hours for fingerlings in both species. Larva and fry were most affected. There was no significance difference (P>0.05) in the effects of AED on both species. The practice of using LLINs for fishing should be discouraged. This negatively affects fish, aquatic biodiversity, and the environment.

Key words: fish, lethal, toxicology, malaria, mortality, insecticide, aquatic biodiversity, clarias gariepinus, heterobranchus bidorsalis

Introduction

Pyrethroids (also known as synthetic Pyrethroids) are insecticides chemically similar to pyrethrins found in pyrethrum extracted from the flower of chrysanthemum, known for centuries for their insecticidal activity.1 They are used as pesticides in homes and for agricultural purpose. Pyrethroids are historically divided into two types, according to their chemical structure. Type I (First generation) pyrethroids, do not contain an α-cyano group in the molecule (for example, allethrin, resmehrin, D-phenothrin, and permethrin), and causes mainly tremors (T-syndrome).2 Type II (Second generation) pyrethroids contain an α-cyano group (for example, deltamethrin, cypermethrin, cyfluthrin, ɣ-cyhalothrin and fervalerate. These groups cause choreoathetosis and salivation (CS-syndrome).2 They also cause paresthesia, which is characterised by transient burning/tingling/itching sensation of the exposed skin.3 The first generation pyrethroids are less toxic to mammals than the second generation.3 Deltamethrin products are among the most popular and widely used insecticides in the world. Deltamethrin was synthesized in 1974, and first marketed in 1977.4 The chemical formula of deltamethrin is C22H10Br2NO3. It is used extensively in agriculture for controlling pest, insects and vectors of endemic diseases, protecting seeds during storage and fighting household insects because of their low environmental persistence.5 Deltamethrin is composed of eight stereometric esters (four cis and four trans isomers) of the di-bromo analogue of chrysanthemic acid, 2, 2–dimethyle-3 cyclopropanecarboxylic acid.4 It is prepared by the esterification of (1R,3R)-or cis2, 2-dimethyl-3 (2,2, dibromovinyl) cyclopropanecarboxylic with (alpha, s)-or (+)-alpha-cyano-3-phenoxybenzyle alcohol or by selective recrystallization of the racemic esters obtained by esterification of the (1R, 3R) –or cis-acid with racemic or (alpha-R, alpha-s, or alpha-R/S)–or + or – alcohol. Deltamethrin is used in the production of Long-lasing Insecticide-treated Nets (LLINs). They have become particularly very prevalent in malaria endemic countries like Nigeria, and are regarded as an important tool in the “Roll Back malaria Campaign”. Thus, it plays a key role in the control of malaria vectors (mosquitos). Deltamethrin is a neurotoxin, and temporary attacks the nervous system of any animal it comes in contact, including human. It cause tingling or reddening of the skin, and has been reported to be responsible for allergen and asthma in some people.6 There was wide-spread repots of isolated fatal incidence associated with the use of LLINs between 2014-2015 in Nigeria. In South Africa, deltamethrin was isolated from breast milk, believed to get in through the skin.7 It is however, generally, considered safe to use around human.6 Reports of acute deltamethrin-human poisoning, other than from occupational exposure, are rare.6 The world Health Organization classified it as ‘moderately hazardous’.8 Nevertheless, deltamethrin is very toxic to aquatic organisms. Deltamethrin and other Pyrethroids have been found to be extremely toxic to fish.9,10 There are several reports on the induced toxic effects of deltamethrin in different fish.11

It has been observed that, because LLINs are distributed freely, local residents sometimes easily use them inappropriately; as fishing nets and for construction of passive fishing gears (traps).12–14 Studies on the effects of deltamethrin on fish abound, and well documented.15–17 Most of the studies used deltamethrin products, particularly pesticides. There is no study on aqueous extracted deltamethrin (AED) from Long-lasting Insecticide-treated Net on members of the family clariidae. Apart from agricultural sources, large volumes of deltamethrin which escaped into the aquatic environment in Sub-Saharan Africa come from the use of LLINs for fishing. The aim of this study is to determine the effects of aqueous extracted deltamethrin on Claris gariepinus (Burchell, 1822) and Heterobranchus bidorsalis (Geoffory Saint-Hilaire, 1809). The study objectives are: To protect and conserve fish, and other aquatic organisms from the neurotoxicity of pyrethroids (deltamethrin), to discourage the use of LLINs for fishing, thus sustaining efforts to control malaria.

Materials and methods

Test organisms

360 Clarias gariepinus and 360 Heterobranchus bidorsalis fish seeds used for the study. They were obtained from a standard fish hatchery. The fish seeds where of same age from each group (larva, fry and fingerlings). The fish were used in accordance with the Canadian guidelines on the care and use of fish in research, teaching and testing18 and National Research Council guide for the care and use of laboratory animals.19

Aqueous extraction of deltamethrin from LLINs

Three new LLINs with a trade name Dawaplus 2.0 freely distributed under the auspices of the US president malaria initiative, produced by Tana nettings in Pakistan were used for the extraction. The nets were exposed to air, free from sunlight and rain for 21days, prior to extraction. The nets were then soaked in a plastic bowel with 15litres of clean water. The physicochemical parameters of the water were: 6mg/l, 26°c and 215mg/l for dissolved oxygen, temperature, pH and total hardness respectively. The nets were left soaked in the water for 3 hours after which, they were removed, gently squeezed, and air-dried. The aqueous extracted deltamethrin (AED) was turned into a clean gallon and covered for use.

Experimental design

The experiment consisted of six treatments. Treatment I (0ppt), Treatment II (50ppt), Treatment III (100ppt), Treatment VI (150ppt), Treatment V (200ppt), and Treatment VI (250ppt). Duplicated for C. gariepinus and H. bidorsalis and replicated in pairs. Thus, total replicates were 72. Total number of test organism used was 720, 10 per each replicate.

Exposure of test organisms to AED

Water with the same physicochemical parameters 6mg/l, 26°c and 215mg/l for dissolved oxygen, temperature, pH and total hardness respectively, was introduced into 72 plastic bowls. AED was then added to the bowls to obtain a concentration of 0ppt, 50ppt, 100ppt, 150ppt, 200ppt, and 250ppt. Thus every set of 12 bowels had the same AED concentration. Ten test organisms were introduced in every bowel. Observations were noted and mortality was recorded at 24, 48, 72, and 96 hours of exposure.

Data analysis

Mortality rate (%) was calculated using the expression:
Mortality rate (%) = No of dead fish x 100
No of fish exposed to AED
Experimental data was subjected to Analysis of Variance at 95 % confidence level.

Results

100% mortality was observed at 250 ppt from both species at all stages in 48-96 hours. Lowest lethal concentration LC was 50 ppt for larva and fry stages of both species at 72 hours, and 100 ppt at 96 hours for fingerlings in both species. Larva and fry were most affected. Mortality of larvae and fry started within 3 hours of exposure at 250ppt. There was no significance difference (P<0.05) in the effects of AED on both species. The mortality rate of C. gariepinus and H. bidorsalis at 0 ppt, 50 ppt, 100 ppt, 150 ppt, 200 ppt, and 250 ppt exposed to AED for 24, 48, 72, and 96 hours is presented in (Table 1-4) shows that the behavioural pattern of the studied species exposed to AED at 96 hours was the same.

24hrs

48hrs

72hrs

96hrs

Dev. Stage

Conc. (ppt)

Mortality rate (%)

Larvae

0

0

0

0

0

50

0

0

10

30

100

10

20

20

40

150

30

40

60

80

200

40

60

80

100

250

80

100

100

100

Fry

0

0

0

0

0

50

0

0

10

10

100

0

10

10

20

150

20

30

50

60

200

30

40

80

100

250

40

100

100

100

Fingerlings

0

0

0

0

0

50

0

0

0

0

100

0

0

0

10

150

10

20

30

50

200

20

30

60

80

250

60

100

100

100

Table 1 Mortality rates of C. gariepinus at different conc. (ppt) of AED at 24, 48, 72 and 96 hours exposure.

24hrs

48hrs

72hrs

96hrs

Dev. Stage

Conc. (ppt)

Mortality rate (%)

Larvae

0

0

0

0

0

50

0

10

20

30

100

10

30

40

40

150

30

50

60

80

200

50

80

100

100

250

90

100

100

100

Fry

0

0

0

0

0

50

0

10

10

20

100

10

20

20

30

150

20

30

60

60

200

40

50

80

100

250

50

100

100

100

Fingerlings

0

0

0

0

0

50

0

0

0

0

100

0

0

10

10

150

10

30

40

60

200

30

40

70

90

250

70

100

100

100

Table 2 Mortality rates of H. bidorsalis at different conc. (ppt) of AED at 24, 48, 72 and 96 hours exposure.

Behaviour

Larvae

Fry

Fingerlings

Opercula movement increased

-

-

++

Loss of equilibrium

+

++

++

Erratic swimming

++

++

+++

Sudden swimming follow by interruption

-

++

++

Circular swimming

+++

++

+

Loss of colour

+

+

+

Table 3 Behavioural pattern of C. gariepinus exposed to AED for 96 hours.

Behaviour

Larvae

Fry

Fingerlings

Opercula movement increased

-

-

+++

Loss of equilibrium

+

++

++

Erratic swimming

+

+

++

Sudden swimming follow by interruption

-

++

++

Circular swimming

++

++

+

Loss of colour

+

+

+

Table 4 Behavioural pattern of H. bidorsalis exposed to AED for 96 hours.

Discussion

Deltamethrin is very toxic to fish as observed because fish is reported to be deficient in enzymes that hydrolyze deltamethrin. Thus, fish have relatively slow metabolism and slow elimination of these compounds.20Also due to their lipophilicity, Pyrethroids have a high rate of gill absorption.21This explains the strong sensitivity of fish to AED exposures. Acute toxicity and neurotoxic effects of deltamethrin was higher in fish larvae and fry than fingerlings. Getlemanian,12 also observed that pyrethroids are more toxic to smaller fish than larger ones. Moore and Waring,22 reported that sub-lethal pyrethroids exposures altered a number of reproductive and early developmental processes in fish.

The experimental organisms exhibited erratic movement, loss of balance, respiratory distress, operculum open and closes at quick succession. De Micco et al.23 reported that low levels of pyrethroids because neurobehavioral affects in young zebra fish Danio rerio. Deltamethrin usually attacks the nervous system of any animal with which it comes into contact. The neurotoxic effects of synthetic pyrethroids (deltamethrin ) is attributed to the blocking of sodium channels and inhibiting the gamma-aminobutyric acid (GABA) receptors in the nervous filament which results in an excessive stimulation of the central nervous system that something can lead to brain hypoxia.24 Deltamethrin is most toxic to fish. It negatively affects aquatic organisms of biological importance.25

Conclusion

Neurotoxin effect of aqueous extracted deltamethrin on C. gariepinus and H. bidorsalis was demonstrated. Acute toxicity of deltamethrin was observed at the lava, fry and fingerling stages of the experimental fish species. Deltamethrin is highly toxic to fish at all ages. But it affects younger fish much rapidly. Artisanal fishermen and local residents should stop using LLINs meant for the control of malaria for fishing or other purpose. This negatively affects fish, aquatic biodiversity and constitutes environmental risk.

Acknowledgements

None.

Conflict of interest

The author declares no conflict of interest.

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