MOJ
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Research Article Volume 6 Issue 1
Can connarus rubber extract inhibit the induction of insulin resistance by hyperglycemia?
Satomi Kawaguchi, Riki Kawasaki, Ryo Murashige, Kayoko Yamazaki, Shin ichiro Kurimoto, Takanori Nakamura, Yu F Sasaki
Faculty of Chemical and Biological Engineering, Hachinohe National College of Technology, Hachinohe, Japan
Department of Pharmaceutical Health Care Faculty of Pharmaceutical Sciences, Himeji Tokyo University, Himeji, Japan
Laboratory of Natural Products Chemistry, Showa Pharmaceutical University, Tokyo, Japan
Received: January 14, 2020 | Published: February 7, 2020
Citation: Kawaguchi S, Kawasaki R, Murashige R, et al. Can connarus rubber extract inhibit the induction of insulin resistance by hyperglycemia? MOJ Toxicol 2020;6(1) DOI: 10.15406/mojt.2020.06.00166
Abstract
Although it would be ideal if the induction of insulin resistance by the persistence of the hyperglycemic state could be prevented by food components that can be consumed daily, to our knowledge, it has not been reported food components that have such effect. Advanced glycation end products (AGE) is involved in the development and progression of diabetic complications. Here, we investigated whether Connarus rubber (CR) extract can inhibit the production of AGE and the induction of insulin resistance. Twenty-four hours prior to the cultivation under serum-free condition, cells were exposed to CR extract and the glucose concentration in the medium was measured every 24 hours. Glucose intake was promoted in the presence of insulin without but not with hyperglycemia condition, showing that insulin resistance was induced in HepG2 cells under hyperglycemia condition. At 1.25 and 2.5µg/mL CR, glucose concentration decreased time-dependently with statistical significance, showing that the induction of insulin resistance was inhibited by CR extract. Furthermore, CR at >1μg/mL suppressed glycation of human serum albumin (HSA) and oxidation of glycated HSA in a concentration-dependent manner. In conclusion, CR is shown to inhibit the chemical reaction of AGE production and the induction of insulin resistance.
Keywords: insulin resistance, glycated HAS, diabetic complications, HepG2 cells, glycated HAS, T2DM, pharmacological purposes, hypoglycemic, diabetic, type-2 diabetes mellitus, connaraceae,
©2020 Kawaguchi, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.