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Public Health

Research Article Volume 7 Issue 6

Nutrient level & its effect on cognition among ASD children in Bangladesh: a cross-sectional study

Jannatara Shefa,1 Khaleda Islam,2 Mohammad Ferdous Ur Rahaman3

1Bangabandhu Sheikh Mujib Medical University (BSMMU), Bangladesh
2University of Dhaka, Bangladesh
3Department of Internal Medicine, Bangabandhu Sheikh Mujib Medical University (BSMMU), Bangladesh

Correspondence: Jannatara Shefa, Research Medical Officer, Institute of Pediatric Neurodisorder & Autism (IPNA), Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh, Tel +8801715254283

Received: September 25, 2018 | Published: November 16, 2018

Citation: Shefa J, Islam k, Rahaman MFU. Nutrient level & its effect on cognition among ASD children in Bangladesh: a cross-sectional study. MOJ Public Health. 2018;7(6):259264. DOI: 10.15406/mojph.2018.07.00249

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Abstract

Background: Autism is a disorder of neural development characterized by impaired social interaction and communication and also by restricted as well as repetitive behavior.

Method: A cross-sectional study. A total of 384 children (age group 1-12 years) with autism were enrolled in this study. The parents were asked to complete the questionnaire regarding the socio-demographic and eating behavior of their children. They were also asked to provide a 24-hour Re-call method by average of three days food diary. IQ assessment was done with Bayley Scales of infant development (BSID), Independent Behavior Assessment Scale (IBAS), Wechsler Intelligence Scale for Children-Revised (WISC-R). Data analysis was done by SPSS version 20.

Result: Energy intake was lower than the RDA in all age group of the autistic children. Mean intake of protein & calcium were higher than the RDA in all age group of the autistic children but in fat intake it was lower in only 10-12y age group. In case of mean intake of iron it was higher than the RDA in all the age group except 7-9 years & 10-12years age group. VitA, thiamine, VitC intake were lower than the RDA. In case of riboflavin & niacin in two age group 1-3 years & 4-6years they were higher than the RDA but in age group 7-9y & 10-12y it was lower than the RDA. Deficient intake of protein, thiamine, niacin & zinc, which was highly significant with low IQ level of the autistic children (P value was .00).

Conclusion: Children with autism exhibited several abnormalities in terms of eating behavior.

Keywords: autism spectrum disorder, IQ, nutrients

Introduction

A neural developmental disorder characterized by impaired social interaction and communication, along with restricted and repetitive behavior is Autism. Autism not only information processing in the brain but also altering the nerve cells and their synapses connect and organize. However how does this occur is not well understood.1 The first appearance of autism starts during infancy or childhood, and generally follows a steady course without remission.2 Most of the symptoms gradually begin after the age of six months, and on established by age two or three years3 and after that it would be continue through adulthood, but most of the time in more muted form.4 It could not be identified by a single symptom, but with a characteristic triad of symptoms it could be easily identified. Sometimes atypical eating, are also common although not essential for the diagnosis.5 Early childhood is a period when children experience new foods, tastes, and textures. Parents of toddlers and young children often describe their children as “picky eaters”, refusing to try or eat a variety of foods. Although picky eating is not uncommon among young children who are typically developing, pickiness in children with autism spectrum disorders (ASDs) may be even more restrictive and may extend beyond the early childhood period.6–8 In a human body brain is the largest organ which need food & drink for development like any other organ. But the linkage between this diet and brain cells yet unrecognized. In so many research it would be clear that for dietary intake brainwork has been depend which ultimately influence our live. These procedures may be started from early infancy of life. For example, 70% of the energy absorbed by a fetus during pregnancy is directed towards brain development. So every stage of life the correlation of diet & brain has been observed. Mental health and well-being varies on significant diet, but there appears to be no point at which diet has no effect. Although there are different researches looking into important aspects of autistic patients, these have not sufficiently covered the issue of nutritional status and the intelligence capacity. From the existing literature indication is found that there is strong connection between autism, nutritional status and the IQ level.

Methodology

This study focuses nutritional status and IQ level of the autistic children at a single point in a specified time. Considering time period and resource availability, cross-sectional analytical (to show association, correlation, regression among different variables study design was most feasible for this study). Since the number of the population was precisely unknown in Bangladesh but presumably which will be few thousands, the minimum sample size was estimated.

The sample size was use: n =  Z 2 pq/ d 2 MathType@MTEF@5@5@+= feaagKart1ev2aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq=Jc9 vqaqpepm0xbba9pwe9Q8fs0=yqaqpepae9pg0FirpepeKkFr0xfr=x fr=xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaqcfaieaaaaaa aaa8qacaWGUbGaaeiiaiabg2da9iaabccacaWGAbWdamaaCaaabeqa aKqzadWdbiaaikdaaaqcfaOaamiCaiaadghacaGGVaGaamiza8aada ahaaqabeaajugWa8qacaaIYaaaaaaa@432D@

 Where, n = required sample size, Z = standardized normal variable, p = proportion of autistic children (unknown), q = 1-p, d = degree of accuracy desired which is set at 5% level. So sample size of 384 was reasonable one. In this respect margin of error was considered 5% and confidence interval 95%. In addition it was ensured that the age of the sample is 1 to 12 years. The study had been conducting among autistic children (age 1-12 years) who were diagnosed by Pediatric Neurologist & Psychologist clinically at selected centers in Bangladesh where patients come from different parts of Bangladesh. Data were collected from outpatient department (OPD) of Institute of Pediatric Neurodisorder & Autism (IPNA), BSMMU, OPD of The Centre for the Rehabilitation of the Paralyzed (CRP) in Dhaka, Savar & also specialized centers like: Autism Welfare foundation, The Society for the Welfare of Autistic Children (SWAC), Autistic Children’s Welfare Foundation, Bangladesh Jatio Protibondhi Unnoyon Foundation (JPUF), Dhaka. All those hospitals & centers are working with children of autism spectrum disorder. In those centers autism screening, diagnosis, psychological test, management & treatment are given.

Study questionnaire

For demographic data collection part of the questionnaire was drafted by the researcher with the help of supervisor & other experts of INFS, University of Dhaka. The Bangla translation of questionnaires also had been drafted at that time. The questionnaire used for the study contains 8 modules and a total of 35 questions. Anthropometric measurement module was prepared for measuring the height/length, weight of children of age 1 to 12 years. For measuring height/length local made wooden stadiometer was used, for taking weight digital weighing scale (Bathroom scale) was used. IQ assessment tools used by the psychologist were: Bayley Scales of infant development (BSID II), Independent Behavior Assessment Scale (IBAS), Stamford- Binet Intelligence Scale, Wechsler Intelligence Scale for Children-Revised (WISC-R)

Study subject

The children (age 1-12 years) who were diagnosed by Pediatric Neurologist and Psychologist clinically as a children of Autism Spectrum Disorder (age 1-12 years) at OPD of Pediatric Neurology Department was study subject. ASD children of different specialized centers were also taken as a study subject. It would be observed that most of the urban, educated parents were coming with their children by recognizing symptoms of autism. But now-a-days people in low socio-economic status also come to the service provider for diagnosis & management of the children with autism but they are poor in number.

Study procedure

After approval of the study protocol by Dhaka University syndicate and getting permission from respective department, the study was conducted. Children (age 1-12 years) who were referred or come individually in to the OPD of different hospitals diagnosed as ASD were included in the study. After taking written informed consent from the parents (or legal guardian, attendant), pre-tested questionnaires were fill-up, IQ level were collected from psychologist during data collection & height-weight were also measured by the researcher own. The module on “24 Hours Recall Method” (which is universal) contains 5 items of questions where answer would be comprises of different food items. Keeping consistence with the general and specific objectives of the study and terms of reference, the draft questionnaires for nutritional assessment was designed based on the base questionnaires of INFS food data analysis software. The questionnaire was pre-tested on OPD of IPNA, BSMMU at first.

Statistical analysis

To analyze the data Statistical Package for Social Science (SPSS) version 21.0 was used. After entry, range and consistency were checked. Statistical analysis was done by using descriptive statistics. Continuous variables were presented as mean values ± standard deviation (SD), and categorical variables were presented as percentages. For data appropriateness significance test (T test, chi-square test, correlation test and coefficient of variation) was done. Food data were analyzed by software of INFS, University of Dhaka. After macro & micro-nutrient analysis that were compared with demographic data. Data were presented by table and charts. IQ level was measured in different psychological test by registered psychologist, which were also compared with nutritional status & food intake. Data were presented by table and charts. IQ level was measured in different psychological test by registered psychologist, which were also compared with food intake.

Ethical consideration

An ethical permission was taken from IRB of Dhaka University. The study was conducted maintaining all possible ethical considerations. Informed written consent was obtained before data collection. Informed consent was taken from all contain purposes, methods and benefits of the study. During interview, all sorts of privacy of the data providers were maintained. Confidentiality of data was ensured strictly. Data were preserved in computer and will be used only for the purpose of this research.

Findings of the study

After analysis it was found most of the children (37%) were in the 37-71 months of age group, 26% were 72-107 months, 20% were in 108-120 months & 17% were in <36 months. It was observed in case of autism male children were more affected than female children. There were 82% male children and female children were 18%.It was revealed that in Bangladesh most of the participants were Muslim, 90% followed by Hindu 8% and Buddhist 2%. Age (in years) distribution of the autistic child’s parents (n=384), mother’s mean age were 25 years where SD 5.10 & father’s age were 33 years where SD 5.58, when the children with autism was born. Regarding the educational level of the mother of the autistic children, 24.7% completed post-graduation, 29.4% completed graduation, 41.7% completed secondary to higher-secondary level & only 4.2% completed primary education level. Most of the parent (51%) had family income 30000-59999 taka monthly, 27.2% had <30,000 taka & 22% parents had monthly income 60,000taka & above (Table 1). In Table 2 number of autistic children who underwent psychological test was 384. Among them more than 63% autistic children had IQ level <69 (known as significantly delayed performance by standard method), 24% had IQ level 70-85 (known as moderately delayed performance) & nearly 12% of the children had IQ level 85-114 (known as mildly delayed performance). In case of psychological test 94% were done by BSID II (Bayley Scales of infant development), 3% were done by WISC-R (Wechsler Intelligence Scale for Children-Revised) & 3% were done by IBAS (Independent Behavior Assessment Scale) (Table 2). In Table 3 mean per capita food intake in different age group was calculated. Age group 1-3y, 4-6y,7-9y,10-12y had consumed cereal 189gm, 155gm, 168gm & 179gm respectively. Age group 1-3y, 4-6y,7-9y,10-12y had consumed rice 132gm, 102gm, 120gm & 131gm respectively. Age group 1-3y, 4-6y,7-9y,10-12y had consumed potatoes 5gm, 09gm, 12gm & 13 gm respectively. Age group 1-3y, 4-6y,7-9y,10-12y had consumed pulse & nut 3gm, 7gm, 8gm & 10gm respectively. Age group 1-3y, 4-6y, 7-9y had consumed vegetables 8gm, 18gm, 22gm & age group 7-9y had 19gm consumption. Age group 1-3y, 4-6y,7-9y,10-12y had consumed fruits 20gm, 21gm, 22gm & 18gm respectively. Age group 1-3y, 4-6y,7-9y, 10-12y had consumed poultry 12gm, 25gm, 42gm & 64gm respectively. Age group 1-3y, 4-6y,7-9y,10-12y had consumed fish 37gm, 58gm, 53gm & 44gm respectively. Age group 1-3y, 4-6y,7-9y,10-12y had consumed milk & milk products 130 gm, 133gm, 75gm & 40gm respectively. In Table 4 mean per capita consumption of different kind of nutrient were calculated. Age group 1-3y, 4-6y, 7-9y, 10-12y had consumed energy 852.8kcal, 804.8kcal, 845.6 kcal & 871.3 kcal respectively. Except 1-3 years age group, energy intake was lower than the RDA in all age group of the autistic children. Mean intake of protein & calcium were higher than the RDA in all age group of the autistic children but in fat intake it was lower in only 10-12y age group. In case of mean intake of iron were higher than the RDA in all the age group except 7-9 years & 10-12years age group, which were 9.1 mg & 10.5mg lower than the RDA. Vit A, thiamine, VitC intake were lower than the RDA. In case of riboflavin & niacin in two age group 1-3 years & 4-6years were higher than the RDA but in age group 7-9y & 10-12y it was lower than the RDA.

Relation between children’s IQ level with nutrient intake about all nutrients were not associated with IQ level except protein, thiamine, niacin & zinc, which were highly significant with IQ level of the autistic children (p value was 0.00) where CI was 95% & (P < 0.05) (Table 5).

Baseline characteristics

ASD(%) N=384

Children’s age group

<36 months

65 (17)

37-71 months

142 (37)

72-107 months

100 (26)

108-120 months

77 (20)

Children’s sex

Male

315 (82)

Female

69 (18)

Religion

Muslim

346 (90)

Hindu

30 (8)

Buddhist

8 (2)

Parental age

Mother’s age

15-19 years

63 (16)

20-24 years

81 (21)

25-29 years

156 (41)

>30 years

84 (22)

Father’s age

21-30 years

104 (27)

31-40 years

242 (63)

>40 years

38 (10)

Mother’s education

Primary

16 (4.2)

SSC & HSC

160 (41.7)

Graduation

113 (29.4)

 

Post-graduation

95 (24.7)

Family monthly income

<30,000 taka

104 (27.2)

30,000-59,999 taka

195 (50.8)

60,000 & above

85 (22.0)

Table 1 Baseline characteristics of the study subjects (n=384)

IQ level

Frequency

Percentage

<69 (significantly delayed performance)

243

63.30%

70-85 (moderately delayed performance)

94

24.50%

85-114 (mildly delayed performance)

4

12.20%

Psychological test

Frequency

Percentage

BSAID (age 1-3.5yrs & no speech, no performance)

364

94

WISC-R (6-15yrs, with speech & performance)

10

3

IBAS (2-9 yrs, no speech & no performance)

10

3

Table 2 IQ level of the study subjects (n=384)

Food
Food items
f

 1-3y

4-6y

 7-9y

10-12y

1313T13otal

Mean
(gm)

SD

Mean
(gm)

SD

Mean
(gm)

SD

Mean
(gm)

SD

Mean
(gm)

SD

Cereal

189

165

155

75

168

59

179

61

169

94

Rice

132

169

102

74

120

61

131

63

118

95

White potato

5

18

9

27

12

23

13

25

10

24

Pulse & nut

3

4

7

24

8

16

10

21

7

19

Vegetable

8

37

18

41

22

41

19

49

17

42

Fruits

20

40

21

34

22

32

18

25

21

33

Poultry

12

35

25

47

42

63

64

94

34

63

Eggs

21

32

18

21

17

33

14

19

18

27

Fish

37

41

58

71

53

46

44

36

50

55

Milk product

130

140

133

152

75

115

40

73

99

134

Total food

436

166

461

176

436

166

413

151

441

168

Table 3 Distribution of ASD children by Per capita food consumption (by age) (n=384)

Nutrients

1-3y,71

4-6y,138

7-9y,109

10-12y,65

M

RDA

%

M

RDA

%

M

RDA

%

M

RDA

%

Energy(kcal)

852.8

754

++

804.8

1094

74%

845.6

1375

61%

871.3

1591

55%

Protein (g)

38.3

19.9

++

44.5

31.0

++

46.1

37.1

++

48.0

44.4

++

Fat(gm)

6.8

2.5

++

7.2

6.5

++

7.4

7.8

+

6.8

9.0

76%

Ca (mg)

419.2

240

++

592.4

226

++

492.7

269

++

364.6

301

++

iron (mg)

9.3

4.6

++

9.4

8.3

+

9.1

10.3

88%

10.5

11.5

91%

Vit.A (IU)

619.2

1245

50%

423.5

1099

39%

540.2

1360

40%

1050

1877

56%

Thia (mg)

0.3

.38

79%

0.4

0.65

62%

0.5

0.87

57%

0.5

1.04

48%

Ribo (mg)

0.3

.23

++

0.3

.31

97%

0.3

0.49

61%

0.3

0.44

68%

Niacine (mg)

6.1

4.8

+

7.1

10.0

71%

8.9

13.5

66%

10.8

16.0

68%

Vit.C (mg)

12.4

17.9

69%

15.7

25.3

62%

15.3

27.6

55%

13.8

33.5

41%

Table 4 Comparison of Nutrients intake and age group with RDA

Nutrients name

IQ level

(ANOVA table) significance
P < 0.05

Below 69

69 & above

Mean

SD

Mean

SD

Energy (kcal)

816.1

365.2

263.8

836.9

0.11

Protein (g)

41.9

20.9

24.9

44.4

0.00

Fat (g)

6.99

5.03

5.23

7.13

0.46

CH (g)

146.7

81.4

49.3

148.8

0.46

Ca (mg)

505.2

377.2

545.9

492.8

0.48

Iron (mg)

9.77

11.39

8.10

9.47

0.46

Vit A (IU)

511.8

1338.3

2042.9

600.2

0.16

Carotein
( U gm)

1769.0

5058.4

3725.3

1727.0

0.82

Thia (mg)

.41

.21

.19

.45

0.00

Ribo (mg)

.27

.20

.22

.28

0.11

Niacine (mg)

7.21

4.58

4.73

8.04

0.00

VitC (mg)

14.28

13.62

16.32

14.76

0.40

Zinc (gm)

312.4

157.0

155.7

339.2

0.00

Table 5 Association between nutrients and IQ level of autistic children (n=384)

Discussion

Children with autism spectrum disorder (ASD) have so many problems. Of them atypical food intake and problems of food selectivity take them at risks of nutritional deficiencies. In one of case-control study, it would be found that comparison between ASD & normal children’s anthropometric measurement and food intake, the body mass indices were below of ASD than normal children. In case of food intake it was also observed that there was limited food variety and inadequacy of some intakes which ultimately made them malnourished.9 Our results showed that age group 1-3y, 4-6y,7-9y,10-12y had consumed cereal 189gm, 155gm, 168gm & 179gm respectively. Age group 1-3y, 4-6y,7-9y,10-12y had consumed rice 132gm, 102gm, 120gm & 131gm respectively. Age group 1-3y, 4-6y,7-9y,10-12y had consumed potatoes 5gm, 09gm, 12gm & 13 gm respectively. Age group 1-3y, 4-6y,7-9y,10-12y had consumed pulse & nut 3gm, 7gm, 8gm & 10gm respectively. Age group 1-3y, 4-6y, 7-9y had consumed vegetables 8gm, 18gm, 22gm & age group 7-9y had 19gm consumption. Age group 1-3y, 4-6y,7-9y,10-12y had consumed fruits 20gm, 21gm, 22gm & 18gm respectively. Age group 1-3y, 4-6y,7-9y, 10-12y had consumed poultry 12gm, 25gm, 42gm & 64gm respectively. Age group 1-3y, 4-6y,7-9y,10-12y had consumed fish 37gm, 58gm, 53gm & 44gm respectively. Age group 1-3y, 4-6y,7-9y,10-12y had consumed milk & milk products 130 gm, 133gm, 75gm & 40gm respectively. Relation between children’s IQ level with nutrient intake about all nutrients were not associated with IQ level except protein, thiamine, niacin & zinc, which were highly significant with IQ level of the autistic children (p value was 0.00). Based on the 2002 National Survey of Resident Nutritional Status in China, the rates of inadequacy intake and the borderline inadequacy intake were 9•1 and 41•8% for vitamin A, respectively; the dietary Ca intake was 238 mg/d, which is only one-third of the adequate intakes in Chinese children below 6 years of age.10 Several studies have also found that children with autism consume significantly lower amounts of Ca than those without autism.11–13 Based on the detected serum biochemical assessment, serum vitamin A and Ca levels in children with autism were significantly lower compared with those in children without autism (the serum Ca level of children with autism was also less than the reference range). A previous study reported that no significant difference is observed in the serum Ca levels between children with and without autism, and the detected values are within the normal reference range.14 However, the serum Ca level is increased when the Ca deposited in the skeleton is mobilized, thereby resolving Ca inadequacy. The proportion of Zn intake inadequacy in Chinese children is approximately 50%.15 Zn deficiencies may be related with the characteristics of the Chinese diet, in which cereals constitute the main staple food. So for this case inadequacy of fruits, the mean vitamin C intake level in that region, children with autism was inadequate & significantly lower than that of the typically developing children. No differences were observed in vitamin B12 levels between children with and without autism in our study. However, the serum folate levels in children with autism significantly decreased. A recent study has reported that serum homocysteine levels significantly increase and folate and vitamin B12 levels significantly decrease in children with autism compared with those in typically developing controls.16 Some studies have also demonstrated that children with autism exhibit impaired methylation and homocysteine metabolism.17,18 Folate and vitamin B12 have an important function in homocysteine metabolism. Therefore, the folate defect observed in our study may lead to homocysteine accumulation in the body of children with autism. Sometimes it would be suggested that children with autism spectrum disorder were at a higher risk for underweight, eating more legumes, vegetables, fiber, and some micronutrients (traditional Mediterranean diet) but fewer dairy and cereal products, and less iodine, sodium, and calcium than their typically developing peers. It would be also observed differences existed in total energy intake but healthy eating index and food variety score differences were not significant. Sometimes Autism spectrum disorder group had no dietary recommendations for thiamin, riboflavin, vitamin C, or calcium. Risk of inadequate intake of fiber, vitamin E, and sodium was lower in children with autism spectrum disorder than typically developing children. From the research it would be concluded that (1) risk of inadequate intake of some micronutrients in children with autism spectrum disorder and (2) cultural patterns and environment may influence food intake and anthropometric characteristics in autism spectrum disorder.9 There is growing interest in possible dietary involvement in the etiology and treatment of Autistic Spectrum Disorders (ASD). Research has focused on the physiological and behavioral effects of dietary change but has not examined the effect of exclusion diets on nutritional intake. Nutrient intakes fell below the Lower Reference Nutrient Intake (LRNI) in 12 children (32%) for zinc, calcium, iron, vitamin A, vitamin B12 and riboflavin in the no diet group and four children (50%) for zinc and calcium in the diet group. Fruit and vegetable intakes were higher and cereal, bread and potato consumption were lower in those children using gluten and/or casein free diets. No significant differences in the energy, protein and micronutrient intakes were found between the two groups of children.19 Intelligence is a touchy, and complicated, subject when it comes to autism. Dr. Leo Kanner first described the condition named autism 70 years ago and noted that some children were thought to be intellectually disabled. At that time their social, communication and behavior problems lead them the appearance of intellectual disability to a new world which was unaware of autism's existence. American psychiatrists updated their diagnostic manual in 2013; they faced a lot of difficulty with IQ tests and autism. Although the manual says intellectual disability is common in autism, it also difficult that measuring a child's intellectual ability may be complicated by the symptoms of autism. This study got 63% autistic children had IQ level <69 (known as significantly delayed performance by standard method), 24% had IQ level 70-85 (known as moderately delayed performance) & nearly 12% of the children had IQ level 85-114 (known as mildly delayed performance). From 1966 to 1998, studies found that about only one-fifth of the people with ASD functioned in the "normal range" of intelligence, according to a 1999 review.161 But years later, in 2014, a U.S. In that study it would be found almost half of the children with ASD had average or above average intelligence, an IQ score above 85. Less than a third of the children with autism had intellectual disability, and 23% had IQ scores in the "borderline range" from 71 to 85.20 Effective interventions have lessened the severity of children's language and intellectual delays, experts say.21

Conclusion of the study

In this study various aspect of nutritional deficiencies were observed in autistic children in our country. They were exhibited several abnormal eating behaviour. Energy intake was found lower than the RDA. Mean intake of protein & calcium were higher than the RDA in all age group but fat intake was lower in only 10-12y age group. Mean intake of iron were higher than the RDA in all the age group except 7-9 years & 10-12years age group. VitA, thiamine, VitC intake were lower than the RDA in all age grouped. Riboflavin & niacin were higher in two age group 1-3y & 4-6y but those were lower than the RDA in age group 7-9y & 10-12y. Low intake of protein, thiamine, niacin & zinc were related with the low IQ level in autistic child. However all nutrient intake was not related with IQ level.

Recommendations

In order to address the problem of ASD education level of the parents about autism must be increased. ASD problem symptoms & management of Autism Spectrum Disorders should be included in the curriculum of different education level in Bangladesh. The ASD children should be provided with adequate & balanced diet to improve their Nutrient intake & IQ level as it was found that some of the children didn’t get adequate calories and micronutrients. Awareness raising programme should be arranged for the mass population. Electronic & print media can play an important role in this regard as well as community clinics. Study throughout the country will be helpful to explore the overall situation of the ASD children.

Acknowledgements

None.

Conflict of interest

The authors declare no conflict of interest.

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