MOJ
eISSN: 2373-4442
Submit manuscript...
Research Article Special Issue Lymphology & Phlebology
Parvovirus b19 and auto antibodies reactive with ssDNA in lupus disease: bioinformatics analysis and hypothesis
Mirjana Pavlovic, Sharmistha Chatterjee, Anna Kats, Perambur Neelakantaswamy
Received: January 01, 1971 | Published: November 26, 2018
Citation: Pavlovic M, Chatterjee S, Kats A, et al. Parvovirus b19 and auto antibodies reactive with ssDNA in lupus disease: bioinformatics analysis and hypothesis, republic of Yemen. MOJ Immunol. 2018;6(6):281-286. DOI: 10.15406/moji.2018.06.00244
Abstract
In this article, the possible link between Parvovirus B19 infection and Lupus Disease is hypothesized and the validation of possibility checked through structural features of single stranded (ss) viral DNA. The binding sites on ssDNA are thymidine pentamers as it is confirmed by X-ray crystallography data. Five of them are necessary for recognition and three for binding, and the structure of ss Parvovirus B19 found in literature indicates that. We have also found confirmation for this structure using an interactive programming environment MATLAB®, commonly applied in many technical fields for data analysis indicating the base number where we have at least five consecutive T’s. These segments are potential sites for initial anti ssDNA antibody binding and ssDNA hydrolysis. In a further step, we have located the CpG islands, through the online sequence analysis tool CpGPlot/CpGReport with the specific algorithm parameters such as region length (>50 base pairs), GC% (>30%), and observed/expected CpG ratio (>60%). It has been shown that upon viral infection these unmethylated viral CpGs within DNA or synthetic oligodeoxynucleotides (ODNs) can stimulate immune cells via TLR9, leading to production of antibodies against the virus. Since the virus is retained in the body fluids after infection, its re-appearance could initiate the production of anti-ssDNA autoantibodies with not only neutralizing but also hydrolytic activity in order to completely eliminate the viral antigen. Thus, anti-ssDNA autoantibodies in lupus with hydrolytic activity may be of predictive, diagnostic and prognostic significance in this systemic autoimmune disease.
Keywords: Parvovirus B19, Lupus, Anti-ssDNA autoantibodies, DNA hydrolysis, Toll like receptors, Bioinformatics, Erythematosus, Systemic lupus, Open reading frames, Transcription factors, Pathogenesis, Immunoglobulin, Hyperactivity, B-lymphocytes, Hydrolytic activity
©2018 Pavlovic, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.
Citations
Media Partner & Supporters
