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Opinion Volume 2 Issue 6

Cell Culture Technique in Immunology: Considering the In Vivo Influencing Factors

Abdelaziz Ghanemi1,2,3

1Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, China
2Kunming College of Life Science, China
3University of Chinese Academy of Sciences, China

Correspondence: Abdelaziz Ghanemi, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology Chinese Academy of Sciences, No. 32 Jiaochang Donglu, Kunming 650223, China

Received: November 28, 2015 | Published: November 30, 2015

Citation: Ghanemi A (2015) Cell Culture Technique in Immunology: Considering the In Vivo Influencing Factors. MOJ Immunol 2(6): 00063. DOI: 10.15406/moji.2015.02.00063

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Cell culture represents an important aspect of the biological and medical research in both mechanisms studies and pharmakons evaluations.1,2 It allows studying the cellular and molecular aspects within a certain contexts. However, cell-based tests allow the study/ evaluation of parameters within a limited context compared to the In Vivo conditions. Indeed, under the In Vivo conditions the immune system is a part of a network that includes also other systems such as the hormonal system and the nervous system.3 Each one of the systems is in a continuous interaction with the other systems and within the same system continuous interactions have also been reported. These interactions could influence parameters such as cell growth, expression of certain receptors and the metabolic activities.

Therefore, the cell cultures on which immunological studies are based might not be fully extrapolated due to the differences in term of influencing factors between the cell cultures conditions and the In Vivo conditions including the hormones and the neurotransmitters. Thus, adding factors such as immune factors, hormones and neurotransmitters, to mimic the In Vivo conditions, within the cell culture medium used to cultivate the immune cells would better mimic the In Vivo conditions toward a better extrapolation for the obtained results especially with the consideration of the exogenous molecules4,5 that can also influence the cells cultures of the immune cells.

The described concepts, although they have been defined for the cell cultures of the immune cells, are also applicable to the cells cultures derived from systems for which the constitutive cells are in continuous interactions either within the same system or with other systems.


Abdelaziz Ghanemi is a recipient of a 2013 CAS-TWAS President's Postgraduate Fellowship.

Conflicts of interest

Author declares there are no conflicts of interest.




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