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Research Article Volume 13 Issue 2
Some locally fermented milk products sold in Onitsha main market and their bacteriological analysis
Osuji MI, Nkwonta RC
Received: June 18, 2025 | Published: June 30, 2025
Citation: Osuji MI, Nkwonta RC. Some locally fermented milk products sold in Onitsha main market and their bacteriological analysis. J Microbiol Exp. 2025;13(2):49-52. DOI: 10.15406/JMEN.2025.13.00432
Abstract
This research was done to unveil the bacteria associated with three locally sourced fermented milk product sold at Onitsha main market in Anambra state in the South-East of Nigeria. The three locally sourced samples include; Nono, Soybean and Yoghurt. They were obtained aseptically from three different hawkers one for each. Ten-fold serial dilution was done while spread plate technique was adopted. After the inoculation of the second, fourth and six diluents followed by 24 hours incubation. The some of the results obtained include; 6.0 x 104, 2.0 x 104 and 1.0 104 for NONO corresponding to nutrient agar, MaConkey and SS agar plate. For the Soybean sample, 4.0 x 10 4, 1.2 x 104 and 1.0 x 104 corresponding to nutrient agar, MaConkey and SS agar plate. Finally, 5.0 x 10 4, 1,5 x 10 4 corresponding to nutrient agar, MaConkey agar plate. No growth was observed for SS agar. Streaking method was used for purification and isolates were stored in agar slants. Biochemical identification tests done revealed possible organisms like; Kebsiella spp, Bacillus spp, Salmonella spp, Escherichia coli, Shigella sp, Lactobacillussp, Vibrio sp. Further visit to the production and processing place of NONO by the research team revealed why it was more contaminated than other samples. Government should assist the locals to maintain good sanitary condition of the place or outright closure.
Keywords: Nono, soybean, yoghurt, sanitary, contaminations, incubation, inoculation
©2025 Osuji, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.