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Research Article Volume 7 Issue 6
Isolation of Streptococcus pneumoniae from the sputum samples and their antimicrobial resistance in Biratnagar, Nepal
Sanjay Mahato, Hema Kumari Sah, Sudhir Yadav
AASRA Research and Education Academy Counsel, Nepal
Department of Microbiology, Mahendra Morang Adarsha Multiple Campus, Tribhuvan University, Nepal
Received: September 27, 2019 | Published: November 26, 2019
Citation: Mahato S, Sah HK, Yadav S. Isolation of Streptococcus pneumoniae from the sputum samples and their antimicrobial resistance in Biratnagar, Nepal. J Microbiol Exp. 2019;7(6):299-304. DOI: 10.15406/jmen.2019.07.00274
Abstract
Sputum is one of the best specimens for the isolation of respiratory tract infections pathogen. Ten sputum specimens were collected from different hospitals of Biratnagar city where patients of age group 40–73 years suffering from headache, coughing, mild fever, chest pain had visited. In this study, a pathogen of pneumococcal disease was isolated from the sputum sample in 5% goat blood agar by quadrant streak method. The predominant bacterial pathogen isolated from sputum sample was 10% Streptococcus pneumoniae and 90% S. viridian. An old patient of 73 years of age was identified positive with pneumococcal disease with organisms like S. pneumoniae and S. viridian. These pathogens were identified by various biochemical test likewise, catalase, optochin, and bile solubility. S. pneumoniae isolate exhibits high sensitivity against chloramphenicol and amoxicillin but resistance against cefotaxime and erythromycin.
Keywords: sputum, Streptococcus pneumoniae, S. viridian, Biratnagar, resistance
©2019 Mahato, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.