Journal of
eISSN: 2373-437X
Submit manuscript...
Research Article Volume 6 Issue 1
Helicobacter pyloriand Inflammatory Bowel Diseases in Tunisia
Hajer Battikh, Meriem Serghini, Christophe Burucoa, Asma Ben Othman, Meriem Zribi, Ali Mrabet, Azza Filali, Chedlia Fendri
Microbiology Laboratory, Rabta University Hospital of Tunis, Tunisia
Gastroenterology a Unit, Rabta University Hospital of Tunis, Tunisia
Bacteriology Laboratory, Poitiers University Hospital, France
Epidemiology and public health, Medecine Faculty of Tunis, Tunisia
Received: December 28, 2017 | Published: January 18, 2017
Citation: Battikh H, Serghini M, Burucoa C, Othman AB, Zribi M et al. (2018) Helicobacter pylori and Inflammatory Bowel Diseases in Tunisia. J Microbiol Exp 6(1): 00180. DOI: 10.15406/jmen.2017.06.00180
Abstract
Background/Aims: The prevalence of Helicobacter pylori(H. pylori) infection has been reported to be lower in individuals with inflammatory bowel disease (IBD) in both developing and developed countries. We investigated H. pylori infection in Tunisian patients with IBD and looked for possible associations of H. pylori infection and drug therapy for IBD. We also studied multiple and mixed infection by culture and antibiotic susceptibility methods and random amplified polymorphic DNA-PCR.
Methods: We studied 60 Tunisian IBD patients, including 45 CD (Crohn’s disease) and 15 ulcerative colitis (UC) patients. Infection rates of H. pylori IBD patients as detected by serology were compared to control patients. H. pylori strains isolated from fundic and antral biopsies were studied by conventional culture methods. Two to nine single colonies per patient were subcultured so as to obtain pure isolates. Antimicrobial testing was performed for 25 strains. RAPD-PCR was conducted in 42 strains.
Results: The prevalence of H. pylori infection in the IBD patients was lower than the controls (53.3% vs 63.2%, p=0.960) whereas CD patients were observed to have the same rate as UC patients (p=0.618). There was a tendency for a higher frequency of lesions on the small bowel and colon in H. pylori-positive compared with H. pylori-negative CD patients (p=0.447). A history of surgery seems to be more frequent in both H. pylori-negative CD patients (p=0.167) andH. pylori-positive UC patients (p=0.231). For the medical treatment regimens shown, there was no significant difference between H pylori positive and negative. Mixed infection concerned metronidazole and ciprofloxacin in 1 patient while 1 patient showed a discordant susceptibility with regard to metronidazole. RAPD-PCR revealed that 4 of 5 patients displayed identical fingerprint profiles of strains, whereas one patient displayed 2 distinct fingerprint profiles.
Keywords: Crohn’s disease, Helicobacter pylori, Inflammatory bowel disease, RAPD-PCR, Tunisia, Ulcerative colitis, Gastrointestinal, Mucosa, Stomach, Epidemiological, Seroprevalence, Gastric pathology, Serology, Tetracycline, Ciprofloxacin, Erythromycin
©2017 Battikh, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.