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Research Article Volume 12 Issue 3
Response of Phaseolus vulgaris to the leaf endophyte Stenotrophomonas rhizophila at reduced dose of nitrogen fertilizer
Paulina Correa-Flores, Dora Alicia Peréz-González, Neeran Obled Jasmin, Juan Manuel Sánchez-Yáñez
Received: May 27, 2025 | Published: June 12, 2025
Citation: Correa-Flores P, Peréz-González DA, Jasmin NO, et al. Response of Phaseolus vulgaris to the leaf endophyte Stenotrophomonas rhizophila at reduced dose of nitrogen fertilizer. J Appl Biotechnol Bioeng. 2025;12(2):102-106. DOI: 10.15406/jabb.2025.12.00391
Abstract
The healthy growth of Phaseolus vulgaris demands NH4NO3, applied in excess it causes loss of soil productivity and environmental pollution. An alternative solution is to reduce NH4NO3 to 50% and inoculate the seed with an endophytic bacterial genus and species: Stenotrophomonas rhizophila. The objective of this research was to analyze the effect of S. rhizophila on the growth of P . vulgaris and NH4NO3 at 50%. There isolated S. rhizophila from P . vulgaris leaves and then was inoculated into P . vulgaris seeds with NH4NO3 at 50%. Under a randomized block experimental design: 2 controls: P . vulgaris with 100% NH4NO3 uninoculated or relative control (RC), NH4NO3 irrigated only with water or absolute control, P . vulgaris with S. rhizophila and NH4NO3 at 50%. Through the response variables: germination percentage; Seedling phenology: plant height and root length, biomass: fresh and dry aerial weight, fresh and dry radical weight. The numerical values of the results were analyzed by Tukey, p <0.05. The results showed a positive effect of S. rhizophila on the germination of P . vulgaris compared to P . vulgaris uninoculated and NH4NO3 at 100%. A S. rhizophila seedling with NH4NO3 at 50% had a beneficial effect on P . vulgaris in plant height and root length, as well as fresh and dry aerial and radical weight. This data supports that S. rhizophila colonized the interior of the roots of P . vulgaris by converting organic compounds from the root metabolism into phytohormones and optimizing NH4NO3 at 50% Compared to the growth of P . vulgaris uninoculated with at 100%. The above indicates that there is a biological option to conserve soil fertility preventing greenhouse gas, without compromising the healthy growth of P . vulgaris. At the same time avoid any kind of soil pollution due to NO3- excess did not uptake by the root system of the plant. For cropping P. vulgaris under sustainable agriculture.
Keywords: soil, legume roots, nitrogen fertilizer, endophytic beneficial bacteria, sustainable agriculture
©2025 Correa-Flores, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.