Evidence of immune genes in the crinoïd: Antedon bifida evidence of A.bifida Igkappa gene and a FC receptor one

doi:10.15406/jabb.2018.05.00154

Journal of

eISSN: 2572-8466

Applied Biotechnology & Bioengineering

Research Article Volume 5 Issue 5

Evidence of immune genes in the crinoïd: Antedon bifida evidence of A.bifida Igkappa gene and a FC receptor one

Michel Leclerc,¹

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Franck Letourneur,² Dominique Davoult,³ Ariane Jolly,⁴ Pierre de la Grange⁴

¹556 rue Isabelle Romee, France
²INSERM, Plate-forme genomique,Institut Cochin, France
³Universite Pierre et Marie Curie, station biologique de Roscoff, France
⁴Genosplice, France

Correspondence: Michel Leclerc, 556 rue Isabelle Rom?e, 45640 Sandillon, France

Received: July 08, 2018 | Published: October 4, 2018

Citation: Leclerc M, Letourneur F, Davoult D, et al. Evidence of immune genes in the crinoïd: Antedon bifida evidence of A.bifida Igkappa gene and a FC receptor one. J Appl Biotechnol Bioeng. 2018;5(5):303-304. DOI: 10.15406/jabb.2018.05.00154

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Abstract

Immuno-genomics studies realized in Echinodermata (Invertebrates) were surprising. 3 classes out of 5 Echinodermata presented an IGKappa gene and a Fc receptor gene. It was, first demonstrated, in Asterids and Ophuirids. It was, secondly clearly shown, in the ancestral Crinoïd: Antedon bifida

keywords: invertebrates, echinodermata, crinoïds, igkappa gene, fc gene, A. bifida

Introduction

The general idea that emerged from the experiments, made in our laboratory, was that Echinodermata, as exemplified, by sea star Asterias rubens (Asterids)and by Ophiocomina nigra (Ophuirids), possessed an immune system, able to mount cellular and humoral-specific responses. After stimulation with a foreign antigen:the horse-radish peroxydase(HRP).^1,2 Then,these echinodermata produced a primitive antibody, correlated to an Igkappa gene^3–5to a Fab gene,⁶ a Fc receptor gene.⁷ But a question desserves to be put: did the ancestral Echinodermata, Antedon bifida, (Crinoïd) possess such genes? It is why, in a first time, we look for IgKappa gene, Fab gene, Fc receptor gene in this crinoïd by the mean of genomic studies.

Materials and methods

Animals: Antedon bifida was obtained at the station « Of Biologie Marine of Roscoff » France.
Obtention of crinoïd mRNA: Digestive coeca were excised from the A. bifida body. bifida mRNA was obtained from Uptizol (Interchim). Quality control was operated.
Sequencing: Sequencing was made on Illumina Next Seq 500 with paired-end : 2. 75 bp Transcriptome was assembled from RNA-Seq fastq files using Trinity v2.1.1 (⁸with default parameters. A BLAST database was created with the assembled transcripts using makeblastdb application from ncbi-blast+ (v2.2.31+). The sequences of transcripts of interest were then blasted against this database using blastn application from ncbi-blast+ ⁹with parameter word_size 7.
Results: The Table 1 which is following summarizes the found Antedon bifida transcriptomes of Igkappa gene and Fc receptor gene of IgA (FCAR) of IgE (Fcer2a) we met in Homo sapiens and in Mus musculus: The Antedon bifida IgK transcriptome sequence is the one:

>TRINITY_DN9178_c0_g1_i2 (Igk):

5'AGCGAATGAAAAAGAAGAACCGGCCAAAAAAAGTACTTCTACCAAAGAAGCGAATGAAAA
AGAAGAACCGGCCAAAAAAAGTACTTCTACCAAAGAAGAAACTGAAATAGAAGAACTAAC
CGAAACAAGTATTTCTACAAAATCAGTTTCTGCCAGTGATATATTCCTTGGTACAACTTT
CACACTGGAGATGGGATTCTGCGTAGGACCTGAACACAAACCGTTTACAGGAGATTTCGA
CGGTGACGGTAATGAAGATCTTCTGTTTCACAATTCAAAGACAGGCTCGAAAAAGATATA
CTATGCAAGTTGTGACGGCTCTTTTAATGGTGATAGGTCGTGGAGAAGAGAGATGAATTT
TTGCTACGTAAGTGGATATGATCTATACATTGGTGATTTCAACGGCGATGGTCGATCCGA
TATGCTGTGTCATCGTCCTCAGTATGGTCAGATTTGGGTTGTGTTGGCGCAACCTGGGGG
TGTATTCACTGCTAACCCGTGGTCGTATAGTCCCAATTGGTGCAAGGCCACCACTGATAA
AGTATATATTGGAGACTTCAACGCAGACGGTCGGGATGATATTCTTTGCCACACACAAAG
TTCGGGTTACATTGCAATATATTATGCATTATACACTGGTTATTTTTCTACCTCTACAAC
ATATCGCTTTACACGAAGTATGAGTTGGTGCAGAGGTACATATCAAAGAGTGTATACTGG
AGATTTCAACGGAGACCGAAGGGTTGATATGCTCTGCCACGACTACTCATCTGGCTACAT
ATATGTAGCAGTAGCCACAGCGACTGGTGGATTCACCTCTGCCACATGGAGCAGAAGTAT
GGGCTGGTGCAAGCATTCGAACTCTAAGCTCAGCATTGGAGATTTCAATAAAGATAACCG
CGACGACATCATGTGCAGCGACACAAATGGTCCTTACTGGATAGCATTCTCTCTGTACAA
CGGTTCGTTTTCATCTAAAAGCTGGACCCGTAAACAAAACTGGTGTACATCTGGCAATGA
TGTGTTAGTTTCGGATGTGAATGGAGATGGTGGGGATGATTTGATGTGCCATAATGAAGC
CGACGGCATCAAGTACATATCGATCAACCATAAGGCCTAAAGCAAGTTCCTCTCAATATT
ACAGAAAACTATTCACCACAAATGATTCATTTTGTACTGAACCTCAATTCAAATTCAATT
AAAATTTACATAAACGTTAACGGAAGGATACAATCAACTAAAATAATGTTCATTCATTAT
TTTTCGTCGATAACCTAAACAAAAATCAGATAAGAAATTATACAATAATATACTGTAAAC
GTATTATACAAAATAATTAAATGTATATTAAGCTACTGTACTTAGAAATGTACTTGTACG
CTTATTAATATTAATAAGCCTAATGCCCGGGTTGATAATAATAAAATACATTTTTGCAAG
TTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCAAAA
GTCCCAGGCCCCACCCCGACCTACTGAACCAGAAAG3'

The Antedon bifida Fc receptor (FCAR)transcriptome sequence is the one: >TRINITY_DN13535_c0_g1_i1 (FCAR)

5'ACTCTACGAACCAAATTTAAATATAACCCGAGAATGTATGTACAACCGATCCAGTAAAAT
GGTAACATTCAAGACTACGATTATGTACCTTGTTAAATTAAAATAAAATAAAGTACCGGT
AGATACACCATCATAAATATCAGCCTTTCATCAAGCAAAACAGTCATTTGTTTACAGTTC
TGTCTTGAGTAGATGTTCTCAGCAAATTTTTCTAATATAACAATTATAAACTTCTTGTGA
AGATGAAATTTATTGATTTAATTGGATGCCAGTTAATTTTATTTAGAATTATTTTTAATT
TTGATCTGAGAAACAGAATTAAGAACTGGAAAAGAAATGAACAAAAGTTTCCATAAAACT
ATCGTTATTCAATTTAGTTTGTTTCTATACAATGGCAAATTAACACTTTCAAGAGAGGTG
CATATTTTTATTTTTGGTCAACTATGCTTACAATAGGTAAAATAAAATATTAACATCCCC
CTACCCTACTTACATCCCAACTTATCACGTAATAAACCTTATTTCTCTGTCGGGAAAATT
TCAGATTTGAGCAAGTATAATTTTATTTATTATTTCAGAATGTTCTCTATTTTAACTGGG
TGCTTGATACAATTAACATTGTAGAAGTTTATTTGTTTATTTGTTTATTTGTTTGTTTGT
TTGTTTGTTTAGATGGAGTTTCTCTCTTGTTGTCCAGGCTGGAGTGCAATGGCA3'

Query ID	Query Symbol	Species	Subject ID	Identity (%)	Length	Mis-match	Gapopen	Query cover (%)	E-value
BC032451.1	Igk	Homo sapiens	TRINITY_DN9178_c0_g1_i2	89,74	78	7	1	8	4,00E-20
NM_133273.3	FCAR	Homo sapiens	TRINITY_DN13535_c0_g1_i1	79,31	87	13	5	4	5,00E-07
NM_001253737.1	Fcer2a	Mus musculus	TRINITY_DN20232_c5_g2_i1	84,31	51	7	1	2	8,00E-05

Table 1 GenBank (IgK, FCAR, Fcer2a)

Discussion and conclusion

Fortunaly, we find again in another Echinodermata, the IgKappa gene, the Fc receptor gene, we found in the Asterid: Asterias Rubens and in the Ophuirid: Ophiocomina nigra. Fab gene was found also in A.bifida, but the e-value was not significant. A considerable role may be assigned to crinoïd IgKappa gene and Fc receptor gene: it is the one of Antedon bifida primitive antibody, but further research is needed in order, to examine and characterize the "immunocytochemical aspects of immunizations" to HRP (Horse-radish peroxydase) or other antigen in Antedon bifida. Nevertheless, for the third time, we find an invertebrate primitive antibody in an Echinodermata: it's a great novelty!