eISSN: 2373-6372 GHOA

Gastroenterology & Hepatology: Open Access
Research Article
Volume 10 Issue 1

Helicobacter pylori infection in hepatitis B virus carriers in Cotonou: epidemiology and socio-demographic factors associated with the co-infection

Jean Séhonou,1,2 Aboudou Raïmi Kpossou,1,2 Jean-Paul Kanvi,1 Comlan N’dehougbea Martin Sokpon,1,2 Rodolph Koffi Vignon,1,2 Akuvi Claude Gildas Adossou1
1Department of Gastroenterology and Hepatology, National University Hospital Center-Hubert Koutoukou Maga (CNHU-HKM), Cotonou, Benin
2Faculty of Health Sciences (FSS), University of Abomey-Calavi, Benin
Received: November 02, 2018 | Published: January 22, 2019

Correspondence: Aboudou Raïmi Kpossou, Department of Gastroenterology and Hepatology, CNHU-HKM Cotonou, Benin, Tel 0022966181939

Citation: Séhonou J, Kpossou AR, Kanvi JP, et al. Helicobacter pylori infection in hepatitis B virus carriers in Cotonou: epidemiology and socio-demographic factors associated with the co-infection. Gastroenterol Hepatol Open Access. 2019;10(1):14‒17. DOI: 10.15406/ghoa.2019.10.00350


Introduction: Infections with hepatitis B virus (HBV) and Helicobacter pylori (H. pylori) are two major public health issues in the world in general, and in Benin in particular. This study aimed to determine the prevalence of H. pylori infection and the socio-demographic factors associated with the co-infection in chronic HBV carriers in Cotonou.

Methods: This cross-sectional descriptive and analytical study was conducted in the hepato-gastroenterology department of the CNHU-HKM from May to August 2017. We included all patients aged 15 years or older, carriers of HBs Antigen (Ag) who were admitted in that period. Detection of H. pylori was done by looking for H. pylori Ag in their stool.

Results: Among the 121 patients who were positive for HBs Ag (mean age 42.3 ± 11.6 years, sex-ratio 2.26), 70 (57.9%) were also positive for H. pylori Ag test in the stool. The level of education was often high (61%), and the socio-economic level was medium for 80 patients (66%). Most lived with more than 2 people under the same roof (an average of 3.15 people) and 54 (44.6%) were in a household of more than 4 people.

No socio-demographic factor was significantly associated with H. pylori infection in HBs Ag carriers: whether it was their age (p = 0.18), their gender (p = 1), their educational level (p = 0.52), the number of people living under the same roof (p = 0.45) or their socio-economic level (p = 0.81).

Conclusion : The prevalence of H. pylori in patients with HBs Ag is relatively high, but appears to be lower than in the general population. However, among these adults, socio-demographic factors seem to play a minor role in the occurrence of the co-infection.

Keywords: Hepatitis B, Helicobacter pylori, co-infection, prevalence, associated factors


Infections with hepatitis B virus (HBV) and Helicobacter pylori (H. pylori) are two major public health issues in the world in general and in Benin in particular.1 Indeed, their respective prevalences in Benin are 9.9%2 and 74%.3

pylori is a bacterium that colonizes the gastric mucosa and is responsible for gastritis, peptic ulcer disease, as well as gastric adenocarcinoma and malt lymphoma.4 Recent studies showed that H. pylori infection is associated with the progression of diseases other than gastrointestinal diseases. This includes hematological, cardiovascular or autoimmune diseases, chronic bronchitis and coronary sclerosis,4–6 but also liver’s diseases.7 H. pylori DNA could be detected in liver samples from patients with chronic liver disease, suggesting that coexistence with H. pylori could worsen a patient's condition.8 Likewise, a case-control study, done in China by Ponzetto A et al.9 in the 2000s, found a high prevalence (89%) of H. pylori in patients with HVB-related cirrhosis compared to 59% in studies conducted on non-cirrhotic patients. In their study on patients with different stages of liver disease, Huang et al.4 showed that the rate of H. pylori infection increased with the progression of the liver disease. They also noted that the infection was associated with the immunopathological disorders and the immunological tolerance of the patients with liver diseases.4

No previous study in Benin has been reported concerning HBV and H. pylori co-infection. The objective of this study is to determine the prevalence of H. pylori infection in patients with CHB and to analyze the factors associated with this co-infection.


Our study was a cross-sectional, descriptive and analytical study with a prospective data collection. It took place over a 4 months period, from May to August 2017, in the Hepato-gastroenterology Clinic of the National University Hospital Hubert Koutoukou Maga (CNHU-HKM) of Cotonou. We included subjects who were at least 15 years old, HBs Ag carriers, and who gave their verbal consent to participate in the study. Patients who received antibiotics less than a month prior to the inclusion date and / or a Proton Pump Inhibitor (PPI) less than 2 weeks were not included in the study.

Socio-demographic data were collected through an interview with each patient, followed by stool collection. The socio-demographic features were: age, gender, area of residence, level of education, socio-economic level, number of persons living under the same roof and number of persons in the household. The H. pylori antigen search was then performed using Helicobacter pylori Antigen Rapid test Kit (Zhuhai.Encode Medical Engineering Co Ltd, China). Stool collection procedures, analysis and interpretation of results were in accordance with the instructions of the rapid test manufacturer.

Afterward, we recorded all the collected information, including the result of the rapid test, on a standardized form. The collected data was entered and statistically analyzed respectively with Excel 8.0 and SPSS 17.0 for Windows. Quantitative variables were expressed as mean ± standard deviation, when the distribution was normal and described using the median and the interquartile range for abnormally distributed ones. Chi-squared analysis or the Fischer’s exact test were used for the variables’ comparisons. A p-value <0.05 was considered statistically significant.


Characteristics of the studied population

A total of 121 patients with chronic hepatitis B (CHB) were included. The male gender was predominant (84 males, 69.4%) with a sex-ratio of 2.26. The mean age was 42.3±11.6 years (range: 18-74 years old). The modal age group was greater than or equal to 40 years (61/121). In this study, 93 (77%) live in urban areas. Overall, 111 (91.7%) patients were educated. 74 (61.1%) reached a high level of education. The socioeconomic level was considered medium in 80 (66.1%) patients, compared to 39 (32.2%) patients who had a low level. On average, there were 3 cohabitants per roof, and 54 (44.6%) out of 121 patients lived with at least 4 cohabitants under one roof.

Prevalence of H. pylori in the studied population

Out of the 121 patients with hepatitis B, 70 were also positive for H. pylori antigen, indicating a prevalence of 57.9%, with a 95% CI = [48.5; 66.8].

Factors associated with H. pylori -HBV co-infection

Data on the study of associated factors are summarized in Table 1. Considering the gender of the 70 patients with the co-infection, 49 (70%) were male compared to 21 (30%) who were female. Gender was not significantly related to H. pylori infection in HBV carriers (p = 1.00).


Univariate analysis            

H. pylori+

H. pylori- 


IC 95%


Age (in years)


1   (50%)

1   (50%)





6   (50%)

6   (50%)


32 (69.6%)

14 (30.4%)


31 (50.8%)

30 (49.2%)



49 (58.3%)

35 (41.7%)





21 (56.8%)

16 (43.2)



52 (55.9%)   

41 (44.1%)





18 (64.3%)

10 (35.7)

Number of cohabitants living under the same roof


4   (28.6%)

10 (71.4%)





25 (47.2%)

28 (52.8%)


22 (40.7%)

32 (59.3%)

Level of education

Secondary / University 

61 (58.6%)

43 (41.4%)




Unschooled / Primary

9   (52.9%)

8   (47.1%)

Socio-economic condition

Medium / High

48 (57.1%)

36 (42.9%)






22 (59.5%)

15 (40.5%)

Table 1 Sociodemographic factors and association with H. pylori -HBV co-infection.

Furthermore, there was no association between the patient age and H. pylori-HBV co-infection (p = 0.18).

Although the percentage of patients with the co-infection, was higher in those living in rural areas compared to those living in urban areas, the difference was not statistically significant (p = 0.52). The percentage of patients with H. pylori infection who had at least a middle or high school level (87.1%) was higher than those who attended only primary school (12.9%). However, there was no statistically significant relationship (p = 0.83) between the level of study and H. pylori infection in HBV carriers.

Also the number of subjects living under the same roof, as well as the socio-economic level were not significantly related to H. pylori-HBV co-infection (respectively p = 0.45 and p=0.81).


pylori infection can be diagnosed by both invasive and non-invasive methods. Diagnosis based on endoscopic biopsy is the gold standard. But the latter is invasive and is subject to sampling errors because H. pylori tends to be distributed heterogeneously in the stomach.4 The antigen research in the stool is a non-invasive, convenient, inexpensive test that can detect active H. pylori infections. It is based on the use of anti- H. pylori monoclonal antibodies. The sensitivity and specificity of this test are greater than 90%. It is convenient, provides fast results with a positive predictive value (PPV) of 97.1%, a negative predictive value (NPV) of 58% and an accuracy of 91.3%.10 These reasons promote why its application as a diagnosis test for H. pylori infection, especially countries with limited resources such as Benin. We used the antigen research test in our study.

In recent years, researchers have paid particular attention to the relationship between H. pylori infection and liver diseases, especially viral hepatitis.4,7 For these authors, infection with H. pylori would be more common in carriers of viral hepatitis and worsen the prognosis.

In our study, the prevalence of H. pylori among patients with chronic HBV was 57.9% (70/121). This prevalence appeared high but would probably be lower than that of our general population. Indeed, the study based on H. pylori serology, published in 2005 by Aguemon BD et al.3 mentioned a prevalence of 74% of H. pylori in general population. Furthermore a study of Kpossou R et al.11 based on histology, reported a 71.5% prevalence of H. pylori in patients admitted at a digestive endoscopy unit at Cotonou from 2014 to 2015. We could therefore think of a decrease in the frequency of H. pylori infection. This decrease could be explained by the improvement of the living conditions and hygiene of the population, the use of antibiotics for the eradication of H. pylori which becomes more and more common. However, the observed differences in frequency could be also explained by the fact that the studied populations were different and the applied methods for H. pylori diagnosis were not the same.

Fan RQ and Wang C in 2012,12 Huang J, et al. in 20154 and Hao Q, et al. in 201713 reported respectively 64.1%, 58%, and 63.9% of H. pylori infection among patients with chronic liver disease. These results are similar to those of this study. In contrast, Pogorzelska J, et al. in 201714 in Poland, Ponzetto A, et al.9 in Italy noted higher prevalences, which were 67.7% and 89% respectively. This could be explained by the fact that the patients included in the two studies were carriers of HBV at a cirrhosis stage and therefore had an advanced chronic hepatitis B and probably a more collapsed immune system.

Our study found no significant difference between H. pylori infection and socio-demographic factors, including the gender of the patients (p = 1, OR = 1.07), their residence area (p = 0.52, OR = 1.71), their level of study (p = 0.83, OR = 1.26). This could be explained by the fact that infection with H. pylori occurs early in childhood. Actually, by the age of 10, more than 50% of children in developing countries are already infected.15 In 2016, a study conducted at Cotonou, on 102 children from 2 to 18 years old (mean age = 7 years), found a prevalence of H. pylori at 46% (47/102). Factors associated with the presence of H. pylori infection were promiscuity and lack of hand hygiene compliance. In that study too, the infection was not related to sex (p = 0.98), age (p = 0.83) and children's (p = 0.53) or their mothers education level (p = 0.19).16 In contrast, Ding Z17 in China in 2015 reported a significant relationship between H. pylori infection and the area of residence as well as and between H. pylori infection and the socio-economic level. This could be explained by the high rate of cities’ urbanization and the improvement of people's living conditions.


Our study showed that the prevalence of H. pylori infection is relatively high among HBV carriers, but this prevalence is lower than in the general population. It was not associated with any socio-economic or demographic factor. The age of acquisition of these two infections (preferentially in children) could explain these results. This hypothesis could be verified by a similar study in a pediatric population.



Conflicts of interest

The author declares no conflicts of interest.


  1. World Health Organization (WHO) Global hepatitis report 2017. WHO, Geneva, 2017. 83p.
  2. Kodjoh. Situation de la lutte contre les hépatites B et C en Afrique. Médecine et Santé Tropicales. 2015;25:141–144.
  3. Aguemon BD, Struelens MJ, Massougbodji A, et al. Prevalence and risk-factors for Helicobacter pylori infection in urban and rural Beninese populations. Clin Microbiol Infect. 2005;11(8):611–617.
  4. Huang J, Cui J. Evaluation of Helicobacter pylori Infection in Patients with Chronic Hepatic Disease. Chin Med J. 2017;130:149–154.
  5. Matusiak A, Chalubinski M, Broncel M, et al. Putative consequences of exposure to Helicobacter pylori infection in patients with coronary heart disease in terms of humoral immune response and inflammation. Arch Med Sci. 2016;12:45–54.
  6. Wang DZ, Chen W, Yang S, et al. Helicobacter pylori infection in Chinese patients with atrial fibrillation. Clin Interv Aging. 2015;10:813–819.
  7. Wang J, Chen RC, Zheng YX, et al. Helicobacter pylori infection may increase the risk of progression of chronic hepatitis B disease among the Chinese population: a meta-analysis. Int J Infect Dis. 2016;50:30–37.
  8. Rabelo-Gonçalves EM, Sgardioli IC, Lopes-Cendes I, et al. Improved detection of Helicobacter pylori DNA in formalin-fixed paraffin-embedded (FFPE) tissue of patients with hepatocellular carcinoma using laser capture microdissection (LCM). Helicobacter 2013;18(3):244–245.
  9. Ponzetto A, Pellicano R, Leone N, et al. Helicobacter pylori seroprevalence in cirrhotic patients with hepatitis B virus infection [Abstract]. Neth J Med. 2000;56:206–210.
  10. Ibrahim EA, Moustafa MA, Monis W. Comparison between phenol ed chromo-endoscopy and a stool rapid immunoassay for the diagnosis of Helicobacter pylori in patients with gastritis. J Microsc Ultrastruct. 2015;3(4):175–180.
  11. Kpossou R, Vignon R, Saké K, et al. Prevalence of Helicobacter pylori in patients admitted in digestive disease center in Cotonou. Gastroent Hepatol. 2017;71(suppl 2):2S26.
  12. Fan RQ, Wang C. The investigation of Helicobacter pylori in chronic hepatitis patients. Chinese Journal of Gastroenterology and Hepatology. 2012;21(2):163–165.
  13. Hao Q, Sun Y, Zhang J, et al. Helicobacter pylori morbidity in chronic hepatitis B patients : A case-control study. Biome Res India 2017;28(13):5785–5789.
  14. Pogorzelska J, Łapińska M, Kalinowska A, et al. Helicobacter pylori infection among patients with liver cirrhosis. Eur J Gastroenterol Hepatol. 2017;29(10):1161–1165.
  15. Ilboudo D, Sangarel L, Sanou J, et al. Epidemiological and clinical aspects of Helicobacter pylori infection in tropical zone about 150 patients at Ouagadougou National Hospital. Médecine d'Afrique noire. 1997;44(1):24–28.
  16. Sagbo GG, Sehonou J, Padonou C, et al. Factors associated with Helicobacter pylori infection in children in Cotonou (Bénin. J Afr Pediatr Genet Med. 2017;1:33–40.
  17. Ding Z, Zhao S, Gong S, et al. Prevalence and risk factors of Helicobacter pylori infection in asymptomatic Chinese children: a prospective, cross-sectional, population-based study. Aliment Pharmacol Ther. 2015; 42(8):1019–1026.
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