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Volume 2 - Issue 6
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Electrogenic activity and hexavalent chromium reduction by Aeromonas hydrophila CrMFC5
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- One of the main challenges for today's science is to decrease the dependence on the use of nonrenewable energy and the decontamination of natural environments. In this regard, microorganisms could play an important role, since their great capacity for metabolic adaptation, high phenotypic plasticity and rapid reproduction, are key elements in the search of solutions of the previously mentioned problems. The current study evaluated the potential for the reduction of hexavalent chromium associated to biological activity and enzyme activity of chromate reductases using cell-free extract of the electrogenic and chromium resistant strain Aeromonas hydrophila CrMF5. Analysis of the 16S rRNA gene placed the isolate close to the species A. hydrophila. Strain CrMF5 tolerates a chromium concentration of approximately 360mg/L; in the microbial fuel cells, this organism showed electrogenic activity by registering a maximum power density of 7.1mW/m2. 100% reduction of Cr(VI) in 10mg/L bioassays that was reached at 10 hours in LB medium and at 38 hours in industrial wastewater. The cell-free extract showed Cr(VI) reduction activity with optimum values with a temperature of 32ºC and pH of 6.0, in the presence of NADH. The results suggest a good potential for the production of electrical energy in microbial fuel cell and bioremediation processes of water contaminated with hexavalent chromium, particularly when concerning detoxification of industrial wastewater. &source=medcraveonline" title="Linked In">
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