Research Article Volume 7 Issue 4
Department of Statistics, Pondicherry university, India
Correspondence: Subramani J, Department of Statistics, Pondicherry University, RV Nagar, Kalapet, Puducherry?605 014, India
Received: April 13, 2018 | Published: July 20, 2018
Citation: Subramani J. On circular systematic sampling in the presence of linear trend. Biom Biostat Int J. 2018;7(4):286-292. DOI: 10.15406/bbij.2018.07.00220
The present paper deals with the computation of the circular systematic sample mean and its variance in the presence of linear trend among the population values. As a result, explicit expression for the variance of circular systematic sample mean is obtained for a pre-assigned fixed sample size and the population size .The efficiency of circular systematic sampling with that of simple random sampling without replacement is assessed algebraically and also for certain natural populations. It is observed that circular systematic sampling performs better than the simple random sampling without replacement.
Keywords: circular systematic sampling, linear systematic sampling, linear trend, optimal circular systematic sampling, simple random sampling, trend free sampling, yates type end corrections
LetU=(U1,U2,…,UN)U=(U1,U2,…,UN) be a finite population with distinct and identifiable units and YY be a real variable with value YiYi measured on giving a vector of measurements Y=(Y1,Y2,…,YN)Y=(Y1,Y2,…,YN). If Yi=a+ib, i=1, 2, …, NYi=a+ib, i=1, 2, …, N then the population is called a labelled population with a perfect linear trend among the population values. The problem is in general, to estimate the population mean ˉY=1N∑Ni=1Yi¯¯¯Y=1N∑Ni=1Yi on the basis of a sample of size selected from the finite population UU . Any ordered sequence S=(u1,u2,…,un)=(Ui1,Ui2,…,Uin)S=(u1,u2,…,un)=(Ui1,Ui2,…,Uin) 1≤i1≤N1≤i1≤N , and 1≤l≤n1≤l≤n is called a sample of size . Several sampling schemes like, simple random sampling without replacement, systematic sampling are available in the literature for selecting a sample of fixed size nn from a finite population of size NN . For the case of finite population with a linear trend among the population values and N=knN=kn , the linear systematic sampling (LSS) is normally recommended for selecting a random sample of fixed size . Further it is shown algebraically that the estimator from linear systematic sampling is better than the estimator provided by simple random sampling without replacement in the presence of linear trend. The performance of systematic sample mean can be improved further by introducing some modifications on the selection of the samples which includes the centered systematic sampling,1 balanced systematic sampling,2 modified systematic sampling3 and also by introducing changes in the estimator itself like, Yates type end corrections.4 In recent times several attempts are made to find an alternative to LSS. In this connection it is worth to note the following works which are alternative to LSS. Diagonal systematic sampling,5 Generalized diagonal systematic sampling,6 ,6 Determinant sampling,8 Modified linear systematic sampling,9 -11 Generalized modified linear systematic sampling,12 Star type systematic sampling,13 Remainder linear systematic sampling,14 Generalized systematic sampling,15 Remainder linear systematic sampling,14 Modified balanced circular systematic sampling,16 Modified systematic sampling by Huang,17 Lahiri,18 Leu & Tsui,19 Sampath & Uthayakumaran,20 Singh & Garg,121 Singh & Singh,22 Uthayakumaran,23 . For further discussions on linear systematic sampling the readers are referred to Cochran,24 , Gautschi,25 Khan et al.,26 ,27 Gupta & Kabe,28 Murthy,29 Singh,30 Sukhatme et al.,31 Fountain & Pathak,32 Mukerjee & Sengupta,33 Murthy & Rao,34 Wu,35 Zinger36 and the references cited there in.
If the population size NN is not a multiple of sample size n(N≠kn)n(N≠kn) then the linear systematic sampling is not applicable for selecting a sample of fixed size .In such situations, circular systematic sampling (CSS) introduced by Lahiri18 cited in Murthy29 provides a constant sample sizenn and the selected units are distinct if and only if NN and kk are relatively prime numbers. However the circular systematic samples are multiple copies of linear systematic samples when N=knN=kn and provides repetition of sampled units whenNN and kk are not relatively prime numbers. As pointed out by Subramani et al.,13 the problems in circular systematic sampling are the following:
Several attempts have been made in the past to get a suitable value for the sampling interval for the given values of population size NN and sample size nn . Murthy & Rao34 has given the choice for kk as “It may be noted that the sample mean is unbiased for the population mean for all values of kk , through the spread of the sample and hence efficiency is better if kk is taken as an integer nearest to N/nN/n . However, if repetition of the same unit in a sample is to be avoided, then it is desirable to take the sampling interval as [N/n][N/n] . It is shown that necessary and sufficient condition for all samples in CSS to have distinct units is that NN and kk are relatively co-prime”.37 Bell house38–40 has suggested that the choice for the sampling interval k=[(N/n)+(1/2)]k=[(N/n)+(1/2)] when N≠(n−1)kN≠(n−1)k and k=(N/n)k=(N/n) when N=(n−1)kN=(n−1)k .
Sengupta & Chattopadhyay33 have proposed the following: “A necessary and sufficient condition for a circular systematic sampling of size, drawn from a population of NN units with sampling intervalkk ,to contain all distinct units is that [N,k]/k≥n[N,k]/k≥n or equivalently, N/(n,k)≥nN/(n,k)≥n where [N,k][N,k] and (n,k)(n,k) denote respectively the least common multiple and the greatest common divisor of NN andkk ”. However it seems there is no theoretical result or empirical study available to justify the choice of k which ensures the efficient estimator or the estimator with minimum variance compared to other choices ofk .”
Recently Subramani et al.,41 and Subramani & Singh42 have made attempts to address the above problems and introduced the optimal circular systematic sampling (OCSS) together with the explicit expressions for its variance and the sampling interval in the presence of linear trend. In OCSS, the choice for the sampling interval k is
kn mod N=±1kn mod N=±1 (1.1)
wherekn mod N=−1kn mod N=−1 represents kn mod N=N−1kn mod N=N−1
For the hypothetical population with values Yi=a+ib, i=1, 2, …, NYi=a+ib, i=1, 2, …, N ,
The variance of OCSS sample mean is given as
V(ˉyocss)=(N−1)(N+1)12n2b2V(¯yocss)=(N−1)(N+1)12n2b2 (1.2)
The variance of SRSWOR sample mean is given as
V(ˉyr) =(N−n)(N+1)12nb2V(¯yr) =(N−n)(N+1)12nb2 (1.3)
Example 1.1: The procedure of obtaining the optimum value of is explained for the fixed values of sample size and the population size .
If N=12N=12
and n=5n=5
then k=7k=7
. That iskn mod N=−1kn mod N=−1
If N=12N=12
and n=5n=5
then k=5k=5
. That is kn mod N=+1kn mod N=+1
The selected OCSS samples, their means, expected value and the variance are given for the sampling intervalk=7k=7 and k=5k=5 in the following Table 1.1 & 1.2:
For both the cases of sampling intervalk=7k=7 and k=5k=5 , it is obtained that V(ˉyocss)=1N∑Ni=1ˉyi2−ˉY2 V(¯yocss)=1N∑Ni=1¯yi2−¯¯¯Y2
=512.7212−6.52=42.72667−42.25=0.476667=512.7212−6.52=42.72667−42.25=0.476667
The value of the variance given above is coincided with the value obtained through the formula given in Table 1.2
Sample Number |
Sample Values |
OCSS Mean |
||||
1 |
1 |
6 |
11 |
4 |
9 |
6.2 |
2 |
2 |
7 |
12 |
5 |
10 |
7.2 |
3 |
3 |
8 |
1 |
6 |
11 |
5.8 |
4 |
4 |
9 |
2 |
7 |
12 |
6.8 |
5 |
5 |
10 |
3 |
8 |
1 |
5.4 |
6 |
6 |
11 |
4 |
9 |
2 |
6.4 |
7 |
7 |
12 |
5 |
10 |
3 |
7.4 |
8 |
8 |
1 |
6 |
11 |
4 |
6.0 |
9 |
9 |
2 |
7 |
12 |
5 |
7.0 |
10 |
10 |
3 |
8 |
1 |
6 |
5.6 |
11 |
11 |
4 |
9 |
2 |
7 |
6.6 |
12 |
12 |
5 |
10 |
3 |
8 |
7.6 |
Table 1.2 OCSS samples and their means for the sampling interval
Further it seems, no attempt is made to derive the explicit expression for the variance of circular systematic sample mean even after 65 years of its introduction for the case of labelled population with a perfect linear trend. As a consequence, the efficiency of circular systematic sampling is not assessed algebraically with that of simple random sampling without replacement.
The points noted above are motivating the present study, which deals with the following:
Circular systematic sampling
As stated earlier, the LSS is not applicable when the population size is not a multiple of sample size for selecting a sample of fixed size whereas the CSS introduced by Lahiri18 cited in Murthy,29 provides a constant sample size . The steps involved in CSS for selecting a sample of size with sampling interval are given below:
Step 1: Arrange the population units around a circle
Step 2: Select a random number such that
Step3: For selecting a circular systematic sample of size select every elements from the random start in the circle until elements are accumulated.
The selected units Ur,Ur+k,Ur+2k,…,Ur+(n−1)kUr,Ur+k,Ur+2k,…,Ur+(n−1)k be the circular systematic sample of size nn for the random start rr . If r+jk>Nr+jk>N then select the item corresponding to {r+jk−N}{r+jk−N}
The variance of the circular systematic sample mean is obtained as given below:
V(ˉycss)=1N∑Ni=1(ˉyi−ˉY)2=1N∑Ni=1ˉyi2−ˉY2V(¯ycss)=1N∑Ni=1(¯yi−¯¯¯Y)2=1N∑Ni=1¯yi2−¯¯¯Y2 (2.1)
Example 2.1: The procedure of obtaining the value of the sampling interval in the case of circular systematic sampling is explained for the fixed values of sample size and the population size .
If and then . That isk=Int(Nn)=Int(125)=2k=Int(Nn)=Int(125)=2
The selected CSS samples, their means, expected value and the variance are given for the sampling interval in the following Table 2.1.
Sample number |
Sample values |
CSS mean |
||||
1 |
1 |
3 |
5 |
7 |
9 |
5 |
2 |
2 |
4 |
6 |
8 |
10 |
6 |
3 |
3 |
5 |
7 |
9 |
11 |
7 |
4 |
4 |
6 |
8 |
10 |
12 |
8 |
5 |
5 |
7 |
9 |
11 |
1 |
6.6 |
6 |
6 |
8 |
10 |
12 |
2 |
7.6 |
7 |
7 |
9 |
11 |
1 |
3 |
6.2 |
8 |
8 |
10 |
12 |
2 |
4 |
7.2 |
9 |
9 |
11 |
1 |
3 |
5 |
5.8 |
10 |
10 |
12 |
2 |
4 |
6 |
6.8 |
11 |
11 |
1 |
3 |
5 |
7 |
5.4 |
12 |
12 |
2 |
4 |
6 |
8 |
6.4 |
Table 2.1 CSS samples and their means for the sampling interval
For the cases of sampling interval and , it is obtained thatV(ˉycss) =1N∑Ni=1ˉyi2−ˉY2V(¯ycss) =1N∑Ni=1¯yi2−¯¯¯Y2
=515.612−6.52=42.96667−42.25=0.716667=515.612−6.52=42.96667−42.25=0.716667
Computation of circular systematic sample means Consider the labelled population with the population values Yi=a+ib, i=1, 2, …, N. Yi=a+ib, i=1, 2, …, N.
The population mean is ˉY=a+[(N+1)2]b¯¯¯Y=a+[(N+1)2]b (2.2)
After a little algebra the circular systematic sample means are obtained as:
ˉyi.=a+[i+k(n−1)2]b, i=1,2,3…N−k(n−1)=L(say)
ˉyi.=a+[i+k(n−1)2−Nn]b, i=L+1, L+2,…,L+k
ˉyi.=a+[i+k(n−1)2−2Nn]b, i=L+k+1, L+k+2,…,L+2k
ˉyi.=a+[i+k(n−1)2−3Nn]b,i=L+2k+1, L+2k+2,…,L+3k
ˉyi.=a+[i+k(n−1)2−(n−1)Nn]b,i=L+(n−2)k+1, L+(n−2)k+2,…,L+(n−1)k (2.3)
Remark 2.1: Since L=N−k(n−1) then L+(n−1)k=N
From the above expressions the sum of the CSS sample means is obtained as
∑Ni=1ˉyi=∑Ni=1[a+ [i+k(n−1)2]b]−Nbn∑n−1j=1∑ki=1j
∑Ni=1ˉyi=Na+(N(N+1)2+Nk(n−1)2)b−kNbn(n−1)2n
1N∑Ni=1ˉyi=a+(N+12)b=ˉY (2.4)
That is, the CSS sample mean is an unbiased estimator for its population mean.
Computation of variance of circular systematic sample mean
For the labelled population and the corresponding CSS sample means defined in Section 2.1, the derivation of the variance of circular systematic sample mean is given below.
Consider 1N∑Ni=1ˉyi=a+(N+12)b=ˉY
=1N∑Ni=1ˉyi2−ˉY2 (2.5)
By substituting the CSS sample means and the population means in the above expression, the variance of CSS sample mean for the labelled population is obtained as
V(ˉycss)=b2N{∑Ni=1[ i+k(n−1)2]2+N2n2∑n−1j=1∑ki=1j2−2Nn∑n−1j=1∑L+jki=L+(j−1)k+1j(i+k(n−1)2)−N(N+1)24}
After a little algebra, the variance of CSS sample mean is obtained as
V(ˉycss)=b2N{N(N+1)(2N+1)6+Nk2(n−1)24+2k(n−1)N(N+1)4+kN2(n−1)n(2n−1)6n2−kN(n−1)[6N+k(n−2)+3]6−N(N+1)24}
By simplifying the above expression one may get
V(ˉycss)={(N−1)(N+1)12−k(n2−1)(2N−kn)12n}b2 (2.6)
Computation of optimum values for the sampling fraction and the variance of circular systematic sample mean We know that the sampling fraction is obtained as or the positive integer closest to . Without loss of generality, let us assume that . That is is the difference between and and . By replacing the values of k in the variance expression, one may get
V(ˉycss)={(N−1)(N+1)12−k(n2−1)(2N−kn)12n}b2
={(N−1)(N+1)12−(N∓d)(n2−1)(2N−(N∓d))12n2}b2
={(N−1)(N+1)12−(N∓d)(n2−1)(N±d)12n2}b2
={(N−1)(N+1)12−(n2−1)(N2−d2)12n2}b2
By simplifying the above expression one may get
V(ˉycss)={(N−1)(N+1)12n2+(n2−1)(d2−1)12n2}b2 (2.7)
The above expression attains minimum at , which implies or .
That is, the optimum variance of CSS sample mean is exactly the same as given in (1.2).
V(ˉyocss)={(N−1)(N+1)12n2}b2
Hence we conclude that the optimum value of the sampling fraction is obtained as stated by Subramani et al.,41 and Subramani & Singh42 as given in (Table 1.1)
Sample number |
Sample values |
OCSS mean |
||||
1 |
1 |
8 |
3 |
10 |
5 |
5.4 |
2 |
2 |
9 |
4 |
11 |
6 |
6.4 |
3 |
3 |
10 |
5 |
12 |
7 |
7.4 |
4 |
4 |
11 |
6 |
1 |
8 |
6 |
5 |
5 |
12 |
7 |
2 |
9 |
7 |
6 |
6 |
1 |
8 |
3 |
10 |
5.6 |
7 |
7 |
2 |
9 |
4 |
11 |
6.6 |
8 |
8 |
3 |
10 |
5 |
12 |
7.6 |
9 |
9 |
4 |
11 |
6 |
1 |
6.2 |
10 |
10 |
5 |
12 |
7 |
2 |
7.2 |
11 |
11 |
6 |
1 |
8 |
3 |
5.8 |
12 |
12 |
7 |
2 |
9 |
4 |
6.8 |
Table 1 OCSS samples and their means for the sampling interval
By comparing the variance expressions for a SRSWOR sample mean (1.3) and a CSS sample mean (2.7) one can easily show that
V(ˉyr)−V(ˉycss)=(N−n)(N+1)b212n−{(N−1)(N+1)12n2−(n2−1)(d2−1)12n2}b2
={(N−n)(N+1)12n−(N−1)(N+1)12n2+(n2−1)(d2−1)12n2}b2
={(N+1)(n−1)(N−n+1)12n2+(n2−1)(d2−1)12n2}b2≥0
That is, the circular systematic sampling is more efficient than the simple random sampling without replacement. i.e. V(ˉycss)≤V(ˉyr)
It has been shown in Section 3 that the circular systematic sampling performs better than the simple random sampling without replacement. However it is not a trend free sampling33 which can be achieved by introducing Yates type end corrections4 as given below:
The modification involves the usual circular systematic sampling but the modified sample mean is defined as
ˉy*css=ˉycss+a(y1−yn) (4.1)
That is, the units selected first and last are given the weights and respectively whereas the remaining units get the weight .By equating for the population with a perfect linear trend, we get the values for from (4.1) as:
Here one may have the following two situations: (i).The random start is less than or equal to and (ii). The random start is greater than .
Case (i). When the random start is less than or equal to
By setting (4.1) is equal to we get
[i+k(n−1)2]+a(y1−yn)=(N+1)2, i=1, 2, …, N−k(n−1)=L
By putting (y1−yn)=i−(i+(n−1)k)=−k(n−1) ,
we get a=2i+k(n−1)−(N+1)2k(n−1), i=1,2,3,…,N−k(n−1)=L (4.2)
Case (ii). When the random start is greater than
Let the random start lies between and
By setting (4.1) is equal to we get
[i+k(n−1)2−jNn]+a(y1−yn)=(N+1)2, i=L+(j−1)k+1 to L+jk
By putting (y1−yn)=i−(i+(n−1)k−jN)=jN−k(n−1) we get
a=n[(N+1)−2i−k(n−1)]+2jN2n[jN−k(n−1)],i=L+(j−1)k+1 to L+jk (4.3)
Remark 4.1: In the presence of a perfect linear trend the modified circular systematic sample mean becomes the population mean and hence the . In this case the circular systematic sampling becomes a completely trend free sampling (See Mukerjee and Sengupta, 1990).
It has been shown in Section 3 that the circular systematic sampling performs well compared to simple random sampling without replacement whenever there exists a perfect linear trend among the population values. However this is an unrealistic assumption in real life situations. Consequently an attempt has been made to study the efficiency of the circular systematic sampling for a population considered by Subramani6 and Murthy.29 The first data were collected for assessing the process capability of a manufacturing process from an auto ancillary manufacturing unit located in Tamilnadu. The data pertain to the measurements taken continuously during the Turning operation performed on the component Torsion bar in Frontier CNC Lathe Machine. The data were collected for estimating the mean value of the outer diameter of the Torsion bar, one of the key components in integrated power steering system. The measurements were taken continuously for the first 50 components produced in a shift. The 50 measurements based on the order of the production are given in Table 3.1. However the first 37 measurements after arranging the data in ascending order are taken to get a linear trend among the population values as given in Table 3.2. The Second data are about the number of workers for 80 factories in a region. However the first 37 measurements of the data are taken to get a linear trend among the population values as given in Table 3.3.
9050 |
9052 |
9050 |
9052 |
9052 |
9056 |
9056 |
9054 |
9056 |
9058 |
9054 |
9054 |
9060 |
9058 |
9060 |
9058 |
9056 |
9058 |
9058 |
9060 |
9062 |
9064 |
9062 |
9064 |
9066 |
9070 |
9068 |
9072 |
9072 |
9070 |
9072 |
9070 |
9070 |
9072 |
9074 |
9076 |
9078 |
9076 |
9076 |
9078 |
9078 |
9078 |
9082 |
9080 |
9082 |
9080 |
9082 |
9086 |
9086 |
9084 |
Table 3.1 Data of outer Diameter of Torsion Bar
9050 |
9050 |
9052 |
9052 |
9052 |
9054 |
9054 |
9054 |
9056 |
9056 |
9056 |
9056 |
9058 |
9058 |
9058 |
9058 |
9058 |
9060 |
9060 |
9060 |
9062 |
9062 |
9064 |
9064 |
9066 |
9068 |
9070 |
9070 |
9070 |
9070 |
9072 |
9072 |
9072 |
9072 |
9074 |
9076 |
9078 |
|
|
|
Table 3.2 The data arranged in ascending order
51 |
51 |
52 |
52 |
53 |
54 |
57 |
60 |
65 |
67 |
68 |
70 |
71 |
73 |
74 |
76 |
78 |
80 |
81 |
85 |
87 |
88 |
92 |
93 |
97 |
100 |
107 |
110 |
113 |
116 |
119 |
121 |
125 |
127 |
127 |
131 |
134 |
|
Table 3.3 Number of workers in first 37 factories (Murthy, 1967, p.228)
The variances of simple random sample mean, circular systematic sample mean and optimal circular systematic sample mean together with the percentage relative efficiencies are obtained and are presented in Table 3.4. The PREs of the proposed estimator (p) with respect to an existing estimator (e) is computed as
PRE(p)=V(e)V(p)x100
N |
n |
Population 1 |
Population 2 |
||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
PRE- |
PRE- |
PRE- |
PRE- |
||||||||
37 |
2 |
30.59 |
15.95 |
15.95 |
191.81 |
100 |
320.89 |
176.87 |
176.87 |
181.43 |
100 |
37 |
3 |
19.81 |
6.03 |
6.03 |
328.49 |
100 |
207.81 |
68.09 |
68.09 |
305.2 |
100 |
37 |
4 |
14.42 |
4.96 |
4.96 |
290.76 |
100 |
151.28 |
35.99 |
35.99 |
420.34 |
100 |
37 |
5 |
11.19 |
3.15 |
2.93 |
381.51 |
107.37 |
117.35 |
25.19 |
22.69 |
517.19 |
111.02 |
37 |
6 |
9.03 |
1.81 |
1.81 |
498.07 |
100 |
94.74 |
15.68 |
15.68 |
604.21 |
100 |
37 |
7 |
7.49 |
1.7 |
1.66 |
451.2 |
102.11 |
78.59 |
13.74 |
12.01 |
654.37 |
114.4 |
37 |
8 |
6.34 |
1.51 |
1.51 |
420.44 |
100 |
66.47 |
13.17 |
10.12 |
656.82 |
130.14 |
37 |
9 |
5.44 |
0.81 |
0.81 |
673.73 |
100 |
57.05 |
7.57 |
7.57 |
753.63 |
100 |
37 |
10 |
4.72 |
1.01 |
0.76 |
620.11 |
132.72 |
49.51 |
10.02 |
5.44 |
910.11 |
184.19 |
37 |
11 |
4.13 |
4.13 |
0.64 |
641.46 |
641.46 |
43.34 |
13.13 |
5.67 |
764.37 |
231.57 |
37 |
12 |
3.64 |
3.64 |
3.64 |
100 |
100 |
38.2 |
4.73 |
4.73 |
807.61 |
100 |
37 |
13 |
3.23 |
3.23 |
0.37 |
865.15 |
865.15 |
33.85 |
5.55 |
3.85 |
879.22 |
144.16 |
37 |
14 |
2.87 |
2.87 |
0.39 |
743.78 |
743.78 |
30.12 |
11.05 |
2.86 |
1053.15 |
386.36 |
37 |
15 |
2.56 |
0.23 |
0.23 |
1095.3 |
100 |
26.89 |
27.12 |
2.68 |
1003.36 |
1011.94 |
37 |
16 |
2.29 |
1.37 |
0.3 |
754.61 |
449.67 |
24.07 |
14.57 |
2.5 |
962.8 |
582.8 |
37 |
17 |
2.06 |
0.62 |
0.21 |
993.24 |
300 |
21.57 |
6.14 |
1.72 |
1254.07 |
356.98 |
37 |
18 |
1.85 |
0.24 |
0.24 |
781.78 |
100 |
19.36 |
1.88 |
1.88 |
1029.79 |
100 |
37 |
19 |
1.66 |
0.21 |
0.21 |
781.13 |
100 |
17.37 |
1.69 |
1.69 |
1027.81 |
100 |
37 |
20 |
1.49 |
0.45 |
0.15 |
990.67 |
298.67 |
15.59 |
4.43 |
1.24 |
1257.26 |
357.26 |
Table 3.4 Comparison of optimal circular systematic sampling, circular systematic sampling and simple random sampling without replacement
It is seen from the table values that the optimal circular systematic sampling performs better than the circular systematic sampling and the circular systematic sampling performs better than simple random sampling in all the cases. In general, it is observed that for the population with a linear trend the following inequality is true. That is, V(ˉyocss)≤V(ˉycss)≤V(ˉyr)
The Author wishes to record his heartiest thanks for the Editor and the Reviewer for their constructive comments, which have improved the presentation of the paper.
Author declares that there is no conflict of interest.
©2018 Subramani. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.
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