Effect of a chromium-fructan complex on glycemic parameters in obese and obese-T2DM/NIDDM rats

January 31, 2022 Gastroenterology & Hepatology No Comments

The incidence and odds ratio of obesity and Type 2 diabetes, a common sequela of obesity, are increasing in incidence to the point of becoming epidemic in Western society. To determine the potential beneficial effects of a chromium-fructan complex (CFC) on glycemic responses of male obese and obese diabetic (T2DM/NIDDM) rats, groups of 3-4month old lean and obese LA/Ntul//-cp and SHR/Ntul//-cp rats (T2DM/NIDDM-rats, n= 6 rats/group) reared normally on Purina #5012 chow from the time of weaning, were subjected to an oral glucose tolerance (OGT) after a brief 4-hour fast. A second group of similarly reared T2DM/ NIDDM rats (n=5) aged one year were also administered the OGT and CFC challenges. The T2DM/NIDDM rats develop IGT, elevations in plasma insulin and amylin concentrations by adolescence and glycosuria, proteinuria, renal disease, cataracts, and impaired nerve conduction velocity typical of T2DM/NIDDM by adulthood. Animals were administered a glucose solution equivalent to 2.5grams/kg BW via intragastric gavage, or the same glucose plus the CFC (2.5g/kg BW, containing 200µg Cr/serving) or the CFC alone over a brief (1-2 minute) duration. Measures of blood glucose (BG) were determined at 30-minute intervals from zero to +120 minutes post gavage via tail bleeding, and the area under the curve (AUC) of each group determined. Final body weights (BW) of obese were greater than lean (p=0.05), and obese-T2DM/NIDDM > Obese rats (p=<0.05) and increased further in T2DM/NIDDM rats with older age. OGT of lean rats remained normal throughout, and CFC resulted in a minor decreasing trend in AUC and in BG concentrations at all time points (p= n.s.) The OGT of obese rats demonstrated significant increases in BG at +60, +90-, and +120-minutes post gavage but remained non-diabetic, and CFC decreased the glycemic increase and AUC by ~50%, similar to the glycemic response off lean littermates. The OGT of young T2DM/NIDDM was significantly impaired and CFC resulted in a 32% reduction in the AUC. The OGT and AUC of OLD T2DM/NIDDM rats were greater than in young rats and were decreased by 25% with CFC. OGT and OGG+CFC were similar at both ages in NIDDM rats. These results are consistent with a CFC-mediated improvement in glycemic control in congenic animal models for obesity and obesity+T2DM/NIDDM and suggest that the CFC complex may be a useful nutraceutical adjunct in the dietary treatment of obesity and other glucose intolerant states as they occur in man and animals.

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